Particulate cadaveric allogenic cartilage system
View Patent ↗The present invention is directed to compositions having at least one neocartilage particle, juvenile cartilage particle or a combination thereof and a matrix, and methods and devices that include the compositions.
1. A cartilage treatment kit comprising:
cadaveric, allogenic human juvenile cartilage particles including viable chondrocytes;
biocompatible chondro-conductive/inductive matrix;
a biocompatible storage solution; and
a sealed, sterile container in which the cartilage particles and storage solution are disposed;
wherein the container is positioned within a sealed, sterile pouch having a hollow interior.
2. The kit of claim 1 , wherein the container comprises:
a tray having a lip surrounding a receptacle; and
a removable cover attached to the lip of the tray for sealing the receptacle.
3. The kit of claim 1 , wherein the storage solution comprises at least one preservative.
4. The kit of claim 3 , wherein the preservative comprises a buffer, an amino acid, a sugar, a salt, a mineral or combinations thereof.
5. The kit of claim 1 , wherein the juvenile cartilage particles are from cadaveric juvenile donors less than fifteen years of age.
6. The kit of claim 5 , wherein the juvenile cartilage particles are from cadaveric juvenile donors less than two years of age.
7. The kit of claim 5 , wherein the juvenile cartilage particles are from cadaveric juvenile donors from about 20 weeks to about 13 years of age.
8. The kit of any one of claim 1 , 3 or 5 , wherein the kit comprises juvenile cartilage particles having a dimension from about one to about three millimeters.
9. The kit of claim 5 , wherein the juvenile cartilage particles are from a cartilage site scored in a grid pattern.
10. The kit of claim 9 , wherein the grid pattern is a square grid pattern.
11. The kit of any one of claim 1 , 3 or 5 , wherein the kit comprises juvenile cartilage particles range in size from about 1 to about 27 mm 3 .
12. The kit of any one of claim 1 , 3 or 5 , wherein the juvenile cartilage particles comprise at least 1×10 6 chondrocytes.
13. The kit of any one of claim 1 , 3 or 5 , comprising at least 60 mg of juvenile cartilage particles.
14. The kit of any one of claim 1 , 3 or 5 , wherein the cartilage particles comprise articular cartilage.
15. The kit of claim 14 , wherein the articular cartilage is harvested from a femoral condyle a tibial plateau or an interior surface of a patella.
16. The kit of claim 1 , wherein the cartilage is distributed throughout substantially all of the matrix or just a portion of the matrix.
17. The kit of claim 1 , wherein the chondro-conductive/inductive matrix comprises fibrinogen, fibrinogen/thrombin, albumin, in-situ forming poly(ethylene glycol) (PEG) hydrogel, fibrin/hyaluronate, fibrin/collagen/hyaluronate, PEG/hyaluronate, PEG/collagen, plasma and protein-based adhesives and sealants, natural adhesives and sealants and any combination thereof.
18. The kit of claim 1 further comprising an osteo-conductive matrix.
19. The kit of claim 18 , wherein the osteo-conductive matrix comprises fibrinogen, fibrinogen/thrombin, fibrin/tri-calcium phosphate, fibrin/collagen/tri-calcium phosphate, fibrin/hyaluronate/tri-calcium phosphate, in-situ forming PEG hydrogel sealants, PEG/tri-calcium phosphate, PEG/collagen, demineralized bone matrix, and any combination thereof.
20. The kit of claim 18 further comprising an associated matrix comprising collagen, polylactic acid (PLA), polyglycolic acid (PGA), and combinations thereof.