Detection and monitoring of liver damage
A method of detecting liver damage in a subject comprises measuring the level of caspase-3 generated cytokeratin-18 fragments in the bodily sample. The level of measuring the level of caspase-3 generated cytokeratin-18 fragments is then correlated with liver disease progression.
1. A method of assessing the degree of liver damage associated with non-alcoholic fatty liver disease in a subject with non-alcoholic fatty liver disease, the method comprising:
obtaining a bodily sample from the subject, wherein the bodily sample is selected from the group consisting of blood, plasma, and serum;
measuring the level of caspase generated cytokeratin-18 fragments in the bodily sample;
correlating the level of caspase generated cytokeratin-18 fragments with the degree of liver damage in the subject;
identifying the subject as having more severe liver damage the higher the measured level of caspase generated cytokeratin-18 fragments in the bodily sample compared to a predetermined value; and
administering to the subject having non-alcoholic fatty liver disease a therapeutic agent based on the identified severity of liver damage.
2. The method of claim 1 , the caspase generated cytokeratin-18 fragments being measured using an enzyme-linked immunosorbent assay (ELISA).
3. The method of claim 2 , the enzyme-linked immunosorbent assay measuring the level of caspase generated cytokeratin-18 fragments in units per liter (U/L), wherein the higher the U/L of caspase generated cytokeratin-18 fragments in the bodily sample the more severe the liver damage in the subject.
4. The method of claim 1 , wherein the higher the measured level of caspase generated cytokeratin-18 fragments in the bodily sample compared to a predetermined value, the more likely the subject has nonalcoholic steatohepatitis (NASH) than steatosis.
5. The method of claims 1 , wherein the higher the measured level of caspase generated cytokeratin-18 fragments in the bodily sample compared to a predetermined value, the more likely the subject has moderate to severe fibrosis than mild fibrosis.
6. The method of claim 1 , wherein a level of caspase generated cytokeratin-18 fragments are measured in the bodily sample and the level of caspase generated cytokeratin-18 fragments are correlated with the degree of liver damage in the subject, wherein the higher the measured level of caspase generated cytokeratin-18 fragments in the bodily sample compared to a predetermined value, the more severe the liver damage in the subject.
7. The method of claim 1 , wherein a level of caspase generated cytokeratin-18 fragments which are cleaved at aspartic acid residue 396 are measured in the bodily sample and the level of caspase generated cytokeratin-18 fragments which are cleaved at aspartic acid residue 396 are correlated with the degree of liver damage in the subject, wherein the higher the measured level of caspase generated cytokeratin-18 fragments which are cleaved at aspartic acid residue 396 in the bodily sample compared to a predetermined value, the more severe the liver damage in the subject.
8. The method of claim 1 , wherein a level of caspase generated cytokeratin-18 fragments which are cleaved at aspartic acid residue 396 are measured in the bodily sample and the level of caspase generated cytokeratin-18 fragments which are cleaved at aspartic acid residue 396 are correlated with the degree of liver damage in the subject, wherein the higher the measured level of caspase generated cytokeratin-18 fragments which are cleaved at aspartic acid residue 396 in the bodily sample compared to a predetermined value, the more severe the liver damage in the subject.
9. A method of assessing the degree of liver damage associated with non-alcoholic fatty liver disease in a subject with non-alcoholic fatty liver disease, the method comprising:
obtaining a bodily sample from the subject, wherein the bodily sample is selected from the group consisting of blood, plasma, and serum;
measuring the level of cytokeratin-18 fragments which are cleaved at aspartic acid residue 396 in the bodily sample;
correlating the level of cytokeratin-18 fragments which are cleaved at aspartic acid residue 396 with the degree of liver damage in the subject;
identifying the subject as having more severe liver damage the higher the measured level of cytokeratin-18 fragments which are cleaved at aspartic acid residue 396 in the bodily sample compared to a predetermined value; and
administering to the subject having non-alcoholic fatty liver disease a therapeutic agent based on the identified severity of liver damage.
10. The method of claim 9 , the cytokeratin-18 fragments being measured using an enzyme-linked immunosorbent assay (ELISA).
11. The method of claim 10 , the enzyme-linked immunosorbent assay measuring the level of cytokeratin-18 fragments which are cleaved at aspartic acid residue 396 in units per liter (U/L), wherein the higher the U/L of cytokeratin-18 fragments which are cleaved at aspartic acid residue 396 in the bodily sample the more severe the liver damage in the subject.
12. The method of claim 9 , wherein the higher the measured level of cytokeratin-18 fragments which are cleaved at aspartic acid residue 396 in the bodily sample compared to a predetermined value, the more likely the subject has nonalcoholic steatohepatitis (NASH) than steatosis.
13. The method of claims 9 , wherein the higher the measured level of cytokeratin-18 fragments which are cleaved at aspartic acid residue 396 in the bodily sample compared to a predetermined value, the more likely the subject has moderate to severe fibrosis than mild fibrosis.
14. A method of assessing the degree of liver damage associated with non-alcoholic fatty liver disease in a subject with non-alcoholic fatty liver disease, the method comprising:
obtaining a bodily sample from the subject, wherein the bodily sample is selected from the group consisting of blood, plasma, and serum;
measuring the level of caspase generated cytokeratin-18 fragments which are cleaved at aspartic acid residue 396 in the bodily sample;
correlating the level of caspase generated cytokeratin-18 fragments which are cleaved at aspartic acid residue 396 with the degree of liver damage in the subject;
identifying the subject as having more severe liver damage the higher the measured level of caspase generated cytokeratin-18 fragments which are cleaved at aspartic acid residue 396 in the bodily sample compared to a predetermined value; and
administering to the subject having non-alcoholic fatty liver disease a therapeutic agent based on the identified severity of liver damage.
15. The method of claim 14 , the caspase generated cytokeratin-18 fragments being measured using an enzyme-linked immunosorbent assay (ELISA).
16. The method of claim 15 , the enzyme-linked immunosorbent assay measuring the level of caspase generated cytokeratin-18 fragments which are cleaved at aspartic acid residue 396 in units per liter (U/L), wherein the higher the U/L of caspase generated cytokeratin-18 fragments which are cleaved at aspartic acid residue 396 in the bodily sample the more severe the liver damage in the subject.
17. The method of claim 14 , wherein the higher the measured level of caspase generated cytokeratin-18 fragments which are cleaved at aspartic acid residue 396 in the bodily sample compared to a predetermined value, the more likely the subject has nonalcoholic steatohepatitis (NASH) than steatosis.
18. The method of claims 14 , wherein the higher the measured level of caspase generated cytokeratin-18 fragments which are cleaved at aspartic acid residue 396 in the bodily sample compared to a predetermined value, the more likely the subject has moderate to severe fibrosis than mild fibrosis.