METHODS AND COMPOSITIONS FOR DIAGNOSING AND MONITORING TRANSPLANT REJECTION
Methods of diagnosing or monitoring transplant rejection, particularly cardiac transplant rejection, in a patient by detecting the expression level of one or more genes in a patient, are described. Diagnostic oligonucleotides for diagnosing or monitoring transplant rejection, particularly cardiac transplant rejection and kits or systems containing the same are also described.
1 . A method of diagnosing or monitoring transplant rejection in a patient, comprising detecting the expression level of a nucleic acid in said patient to diagnose or monitor transplant rejection in said patient wherein said nucleic acid comprises the nucleotide sequence SEQ ID NO: 117.
2 . The method of claim 1 wherein said transplant rejection is cardiac transplant rejection.
3 . The method of claim 1 wherein said expression level is detected by measuring the RNA level expressed by said nucleic acid.
4 . The method of claim 3 , further including isolating RNA from said patient prior to detecting said RNA level expressed by said nucleic acid.
5 . The method of claim 3 wherein said RNA level is detected by PCR.
6 . The method of claim 3 wherein said RNA level is detected by hybridization.
7 . The method of claim 3 wherein said RNA level is detected by hybridization to an oligonucleotide.
8 . The method of claim 7 wherein said oligonucleotide comprises DNA, RNA, cDNA, PNA, genomic DNA, or synthetic oligonucleotides.