Control device, control program, and control method for observation unit, and observation system
Provided is a control device for controlling an observation unit including an observation device for allowing a sample to be observed to acquire an observation image of the sample and an illumination device for illuminating the sample when the sample is observed with the observation device, the control device including a first control unit for controlling the illumination device to adjust an illuminance of the illumination device when a search mode is set for the observation unit, and a second control unit for controlling the illumination device to adjust the illuminance of the illumination device when a time lapse mode is set for the observation unit. Here, the illuminance set for the illumination device by the first control unit by adjusting the illuminance of the illumination device is lower than the illuminance set for the illumination device by the second control unit by adjusting the illuminance of the illumination device.
1. A method for obtaining an observation image of a sample using an observation system, the method comprising steps of:
(a) preparing the observation system; wherein
the observation system comprises an observation unit and a computer;
the observation unit comprises:
a loading table which a container with a sample accommodated therein is to be loaded;
an observation device configured to allow the sample to be observed to acquire the observation image of the sample; and
an illumination device configured to illuminate the sample;
the computer comprises a memory;
(b) loading the container with the sample accommodated therein;
(c1) moving the loading table while the illumination device illuminates the sample, so as to search the sample with the observation unit; wherein
when a shutter speed of the observation unit is set to a lower limit value of a range that can be set and a luminance of the observation image fails to fall within a predetermined range of values, the shutter speed of the observation unit is not changed and the illumination device adjusts an illuminance of the illumination device,
(c2) recording the coordinates of the sample which has been searched in the step (c1);
(d1) moving the loading table on the basis of the coordinate of the sample read from the memory;
(d2) illuminating the illumination device by the illuminance which is greater than the illuminance configured in the illumination device in the step (c1);
(d3) conducting auto focus control of the observation device;
(d4) adjusting the shutter speed of the observation device without changing the illuminance in the illuminating device, when the luminance of the observation device fails to fall within the predetermined range of values;
(d5) acquiring the observation image of the sample using the observation device; and
(e) repeating the steps (d1)-(d5), after a predetermined period lapses.
2. The method according to claim 1 , wherein
in the step (c2), the zoom factor relevant to the sample which has been searched in the step (c1) is recorded in the memory; and
in the step (d5), the observation image of the sample is acquired on the basis of the zoom factor which has been recorded in the memory.
3. The method according to claim 1 , wherein
in the step (c2), the focus position relevant to the sample which has been searched in the step (c1) is recorded in the memory; and
in the step (d5), the observation image of the sample is acquired on the basis of the focus position which has been recorded in the memory.
4. The method according to claim 2 , wherein
in the step (c2), the focus position relevant to the sample which has been searched in the step (c1) is recorded in the memory; and
in the step (d5), the observation image of the sample is acquired on the basis of the focus position which has been recorded in the memory.
5. The method according to claim 2 , wherein
in the step (d2), the illuminance of the illumination device is adjusted on the basis of the zoom factor which has been recorded in the memory.
6. The method according to claim 1 , wherein
in the step (c1), the shutter speed of the observation device is set to the lower limit value of the range that can be set for the observation device.
7. A method for obtaining each observation image of a plurality of samples using an observation system, the method comprising steps of:
(a) preparing the observation system; wherein
the observation system comprises an observation unit and a computer;
the observation unit comprises:
a loading table which a container with a plurality of samples accommodated therein is to be loaded;
an observation device configured to allow the sample to be observed to acquire the observation image of the sample; and
an illumination device configured to illuminate the sample;
the computer comprises a memory;
(b) loading the container with the plurality of the samples accommodated therein;
(c1) moving the loading table while the illumination device illuminates the plurality of the samples, so as to search each sample with the observation unit; wherein
when a shutter speed of the observation unit is set to a lower limit value of a range that can be set and a luminance of the observation image is fail to fall within a predetermined range of the values, the shutter speed of the observation unit is not changed and the illumination device adjusts an illuminance of the illumination device,
(c2) recording the coordinates of each sample which have been searched in the step (c1);
(d1) moving the loading table on the basis of the coordinate of one sample read from the memory;
(d2) illuminating the illumination device by the illuminance which is greater than the illuminance configured in the illumination device in the step (c1);
(d3) conducting auto focus control of the observation device;
(d4) adjusting the shutter speed of the observation device without changing the illuminance in the illuminating device, when the luminance of the observation device fails to fall within the predetermined range of values;
(d5) acquiring the observation image of the one sample using the observation device;
(d6) repeating the steps (d1)-(d5) for each sample; and
(e) repeating the steps (d1)-(d6), after a predetermined period lapses.
8. The method according to claim 7 , wherein
in the step (c2), the zoom factor relevant to each sample which has been searched in the step (c1) is recorded in the memory; and
in the step (d5), the observation image of each sample is acquired on the basis of the zoom factor which has been recorded in the memory.
9. The method according to claim 7 , wherein
in the step (c2), the focus position relevant to each sample which has been searched in the step (c1) is recorded in the memory; and
in the step (d5), the observation image of each sample is acquired on the basis of the focus position which has been recorded in the memory.
10. The method according to claim 8 , wherein
in the step (c2), the focus position relevant to each sample which has been searched in the step (c1) is recorded in the memory; and
in the step (d5), the observation image of each sample is acquired on the basis of the focus position which has been recorded in the memory.
11. The method according to claim 8 , wherein
in the step (d2), the illuminance of the illumination device is adjusted on the basis of the zoom factor which has been recorded in the memory.
12. The method according to claim 7 , wherein
in the step (c1), the shutter speed of the observation device is set to the lower limit value of the range that can be set for the observation device.