Method for diagnosing and predicting cerebellar ataxia
View Patent ↗The present invention relates to an in vitro method for diagnosing and/or predicting hereditary cerebellar ataxia in a dog, and/or identifying a dog which is healthy carrier of hereditary cerebellar ataxia, comprising determining the presence or absence of an homozygous or heterozygous genetic variation in the arylsulfatase G gene sequence in a biological sample from said dog, as compared with the arylsulfatase G gene sequence of a healthy non-carrier dog, wherein the presence of said homozygous genetic variation indicates that said dog is or will be affected by hereditary cerebellar ataxia, and the presence of said heterozygous genetic variation indicates that said dog is healthy carrier of hereditary cerebellar ataxia, said dog being of a breed selected in the group consisting of American Staffordshire Terrier, American Pit Bull Terrier and Pit Bull type.
1. An in vitro method for diagnosing and/or predicting hereditary cerebellar ataxia in a dog, comprising :
obtaining a biological sample from the dog,
determining in the biological sample the presence of a homozygous adenosine allele at the nucleotide position 296 of the arylsulfatase G gene cDNA sequence of SEQ ID NO: 1, and
diagnosing and/or predicting the dog is or will be affected by hereditary cerebellar ataxia when the homozygous adenosine allele is detected,
wherein said dog is selected from the group consisting of American Staffordshire Terrier, American Pit Bull Terrier and Pit Bull type.
2. A method according to claim 1 , wherein the presence of the homozygous adenosine allele is determined by polymerase chain reaction (PCR) and pyrosequencing, by sequencing or by specific amplification of said allele.
3. An in vitro method for identifying a dog which is healthy carrier of hereditary cerebellar ataxia, comprising:
obtaining a biological sample from the dog,
determining in the biological sample the presence of a heterozygous adenosine allele at the nucleotide position 296 of the arylsulfatase G gene cDNA sequence of SEQ ID NO: 1, and
identifying the dog as a healthy carrier of hereditary cerebellar ataxia when the heterozygous adenosine allele is detected,
wherein said dog is selected from the group consisting of American Staffordshire Terrier, American Pit Bull Terrier and Pit Bull type.
4. A method according to claim 3 , wherein the presence of the heterozygous adenosine allele is determined by polymerase chain reaction (PCR) and pyrosequencing, by sequencing or by specific amplification of said allele.