Culture of cells
View Patent ↗The invention relates to a method for culturing human embryonic stem cells (hESCs) and/or induced pluripotent stem (iPS) cells on a lectin. The invention relates also to the use of a lectin in a method for culturing human embryonic stem cells (hESCs) and/or induced pluripotent stem (iPS) celts and a culture medium composition containing a lectin attached on the culturing plates.
1. A method of culturing human induced pluripotent stem (iPS) in an undifferentiated state, wherein a human iPS cell or a human iPS cell population is contacted with at least one lectin immobilized on a surface, wherein said at least one lectin recognizes the structure (Fucα2) n Galβ4GlcNAc, wherein n is 0 or 1, and wherein the human iPS cell or the human iPS cell population is cultured, thereby maintaining the human iPS cell or human iPS cell population in an undifferentiated state.
2. The method of claim 1 , wherein the human iPS cell or the human iPS cell population is contacted with at least one lectin and with a definitive serum-free and feeder-free medium.
3. The method of claim 1 , wherein the human iPS cell or the human iPS cell population is contacted with one lectin.
4. The method of claim 1 , wherein the lectin is ECA, UEA-1, DSA, or galectin.
5. The method of claim 1 , wherein the lectin has the specificity of ECA and recognizes the structure (Fucα2),Galβ4GlcNAc, wherein n is 0 or 1.
6. The method of claim 1 , wherein the lectin is ECA and recognizes the structure (Fucα2) n Gal4GlcNAc, wherein n is 0 or 1, and the human iPS cell population is cultured in either a fibroblast-conditioned media, or bFGF-containing media, thereby maintaining the human iPS in an undifferentiated state.
7. A culture medium composition for culturing induced pluripotent stem (iPS) cells, wherein the composition comprises at least one lectin as a matrix, said iPS cells and a definitive serum-free and feeder-free medium, and wherein the lectin recognizes the structure (Fucα2) n Galβ4GlcNAc, wherein n is 0 or 1.
8. The composition of claim 7 wherein it comprises one lectin.
9. The composition of claim 7 , wherein the lectin is ECA, UEA-1, DSA, or galectin.
10. The composition of claim 7 , wherein the lectin has the specificity of ECA and recognizes the structure (Fucα2) n Galβ4GlcNAc, wherein n is 0 or 1.
11. The composition of claim 7 , wherein the lectin is ECA and recognizes the structure (Fucα2) n Galβ4GlcNAc, wherein n is 0 or 1, said definitive serum-free and feeder-free medium is fibroblast-conditioned media, or bFGF-containing media, and said iPS cells are in an undifferentiated state.