IP Library Granted Patent US 9,060,477
Granted Patent B2
US 9,060,477 · App. 13/005,855 · Granted Jun 23, 2015

Genetic LOCI on maize chromosomes 3 and 4 that are associated with fusarium ear mold resistance

Inventors: Adriana Tomas (Newark, DE); Stanley Luck (Wilmington, DE); Jamie Anne Layton (Newark, DE)
Assignee: E I DU PONT DE NEMOURS AND COMPANY
A01H5/10A01H1/00A01H1/04C12Q1/6895C12Q2600/13C12Q2600/156
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Quick Facts
Patent No.
US 9,060,477
App. No.
13/005,855
Granted
Jun 23, 2015
Kind
B2
Abstract

The invention relates to methods and compositions for identifying and selecting maize plants with enhanced resistance to Fusarium ear mold. Maize plants generated by the methods of the invention are also a feature of the invention.

Claims (37)

1. A method of identifying a maize plant with enhanced resistance to Fusarium ear mold, the method comprising:

a. isolating nucleic acids from a maize plant;

b. analyzing the isolated nucleic acids for the presence of a haplotype associated with the enhanced resistance to Fusarium ear mold, wherein said haplotype is located within a chromosomal interval comprising and flanked by PHM12969 and PHM18211 and comprises:

i. a “C” at PHM12209.11,

ii. a “T” at PHM12209.20,

iii. a “C” at PHM12209.21,

iv. a “G” at PHM12209.22,

v. a “C” at PHM12209.23,

vi. an “A” at PHM9905.11,

vii. a “T” at PHM9905.13,

viii. a “G” at PHM9905.35,

ix. a “T” at PHM2204.88,

x. an “A” at PHM2204.105,

xi. a “C” at PHM13926.25,

xii. a “G” at PHM13926.27,

xiii. a “G” at PHM13926.28, and

xiv. a “G” at PHM13926.32; and

c. selecting the maize plant if the haplotype is detected.

2. A method of selecting a maize plant with enhanced resistance to Fusarium ear mold, the method comprising:

a. identifying a first maize plant that has a haplotype within a chromosomal interval comprising and flanked by PHM12969 and PHM18211, said haplotype comprising:

i. a “C” at PHM12209.11,

ii. a “T” at PHM12209.20,

iii. a “C” at PHM12209.21,

iv. a “G” at PHM12209.22,

v. a “C” at PHM12209.23,

vi. an “A” at PHM9905.11,

vii. a “T” at PHM9905.13,

viii. a “G” at PHM9905.35,

ix. a “T” at PHM2204.88,

x. an “A” at PHM2204.105,

xi. a “C” at PHM13926.25,

xii. a “G” at PHM13926.27,

xiii. a “G” at PHM13926.28, and

xiv. a “G” at PHM13926.32;

b. crossing said first maize plant to a second maize plant;

c. evaluating progeny plants for the haplotype of the first maize plant; and

d. selecting the progeny plants that possess the at haplotype of the first maize plant.

Assignments (2)
NUNC PRO TUNC ASSIGNMENT Recorded Nov 29, 2022
From: E.I. DU PONT DE NEMOURS AND COMPANY
To: CORTEVA AGRISCIENCE LLC
Reel/Frame 063141/0155 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jan 17, 2011
From: TOMAS, ADRIANA; LUCK, STANLEY; LAYTON, JAMIE ANNE
To: E. I. DU PONT DE NEMOURS AND COMPANY
Reel/Frame 025739/0569 →
Continuity (2)
Provisional Application 61298233 · Jan 26, 2010
Related Publication 20110185457A1 · Jul 28, 2011