Genetic LOCI on maize chromosomes 3 and 4 that are associated with fusarium ear mold resistance
The invention relates to methods and compositions for identifying and selecting maize plants with enhanced resistance to Fusarium ear mold. Maize plants generated by the methods of the invention are also a feature of the invention.
1. A method of identifying a maize plant with enhanced resistance to Fusarium ear mold, the method comprising:
a. isolating nucleic acids from a maize plant;
b. analyzing the isolated nucleic acids for the presence of a haplotype associated with the enhanced resistance to Fusarium ear mold, wherein said haplotype is located within a chromosomal interval comprising and flanked by PHM12969 and PHM18211 and comprises:
i. a “C” at PHM12209.11,
ii. a “T” at PHM12209.20,
iii. a “C” at PHM12209.21,
iv. a “G” at PHM12209.22,
v. a “C” at PHM12209.23,
vi. an “A” at PHM9905.11,
vii. a “T” at PHM9905.13,
viii. a “G” at PHM9905.35,
ix. a “T” at PHM2204.88,
x. an “A” at PHM2204.105,
xi. a “C” at PHM13926.25,
xii. a “G” at PHM13926.27,
xiii. a “G” at PHM13926.28, and
xiv. a “G” at PHM13926.32; and
c. selecting the maize plant if the haplotype is detected.
2. A method of selecting a maize plant with enhanced resistance to Fusarium ear mold, the method comprising:
a. identifying a first maize plant that has a haplotype within a chromosomal interval comprising and flanked by PHM12969 and PHM18211, said haplotype comprising:
i. a “C” at PHM12209.11,
ii. a “T” at PHM12209.20,
iii. a “C” at PHM12209.21,
iv. a “G” at PHM12209.22,
v. a “C” at PHM12209.23,
vi. an “A” at PHM9905.11,
vii. a “T” at PHM9905.13,
viii. a “G” at PHM9905.35,
ix. a “T” at PHM2204.88,
x. an “A” at PHM2204.105,
xi. a “C” at PHM13926.25,
xii. a “G” at PHM13926.27,
xiii. a “G” at PHM13926.28, and
xiv. a “G” at PHM13926.32;
b. crossing said first maize plant to a second maize plant;
c. evaluating progeny plants for the haplotype of the first maize plant; and
d. selecting the progeny plants that possess the at haplotype of the first maize plant.