Short cycle methods for sequencing polynucleotides
View Patent ↗The invention provides methods for sequencing a polynucleotide comprising stopping an extension cycle in a sequence by synthesis reaction before the reaction has run to near or full completion.
1. A method for obtaining sequence information from a target nucleic acid, comprising:
a) immobilizing a plurality of different target nucleic acid/primer duplexes at discrete locations on a surface, wherein target nucleic acid portions of said duplexes each comprise the same adaptor sequence, and wherein said primers are hybridized to said adaptor sequence;
b) contacting said plurality of duplexes with a solution comprising a detectably labeled nucleotide that is not a chain terminating nucleotide and an enzyme under conditions wherein the enzyme catalyzes template-dependent addition to a terminus of at least one of said primers, resulting in the addition of a labeled nucleotide to said primer, wherein the label interferes with progression of the enzyme; and
c) detecting the addition to the terminus of said at least one primer,
wherein said addition is indicative of the presence of a complementary sequence in the target nucleic acid.
2. The method of claim 1 , wherein said enzyme is a polymerase.
3. The method of claim 1 , further comprising repeating steps b) and c) one or more times.