Corn event MIR604
View Patent ↗A novel transgenic corn event designated MIR604, is disclosed. The invention relates to DNA sequences of the recombinant constructs inserted into the corn genome and of genomic sequences flanking the insertion site that resulted in the MIR604 event. The invention further relates to assays for detecting the presence of the DNA sequences of MIR604, to corn plants and corn seeds comprising the genotype of MIR604 and to methods for producing a corn plant by crossing a corn plant comprising the MIR604 genotype with itself or another corn variety.
1. A primer pair for detecting the presence of MIR604 nucleic acids in a biological sample, the primer pair comprising a first primer and a second primer designed to bind to a polynucleotide comprising at least 20 contiguous nucleotides of SEQ ID NO: 1 or SEQ ID NO: 2 when said polynucleotide is single stranded, wherein the first primer and the second primer, when used together in a PCR reaction, produce an amplicon that is indicative of MIR604 nucleic acids.
2. The primer pair according to claim 1 , wherein the first primer is designed to bind to a MIR604 insertion sequence and the second primer is designed to bind to a flanking genomic DNA sequence downstream of the 3′ end of the MIR604 insertion site or to a flanking genomic DNA sequence upstream of the 5′ end of the MIR604 insertion site.
3. The primer pair according to claim 2 , wherein the second primer comprises at least 10 contiguous nucleotides of nucleotides 507-1570 of SEQ ID NO: 4.
4. The primer pair according to claim 2 , wherein the second primer comprises at least 10 contiguous nucleotides of nucleotides 1-801 of SEQ ID NO: 3.
5. The primer pair according to claim 2 , wherein the first primer comprises a sequence selected from the group consisting of SEQ ID NOs: 27-36, and the complements thereof, and the second primer comprises a sequence selected from the group consisting of SEQ ID NOs: 39-46, and the complements thereof.
6. The primer pair according to claim 5 , wherein the first primer comprises SEQ ID NO: 27 and the second primer comprises SEQ ID NO: 45.
7. The primer pair according to claim 2 , wherein the first primer comprises a sequence selected from the group consisting of SEQ ID NOs: 16-28, and the complements thereof, and the second primer comprises a sequence selected from the group consisting of SEQ ID NOs: 7-15, and the complements thereof.
8. The primer pair according to claim 7 , wherein the first primer comprises SEQ ID NO: 28 and the second primer comprises SEQ ID NO: 15.