Crystallization media
View Patent ↗A set of crystallization solutions includes a plurality of crystallization solutions of Crystallization Solution Set I, a plurality of crystallization solutions of Crystallization Solution Set II or a plurality of crystallization solutions of Crystallization Solution Set III.
1. A set of crystallization solutions comprising a plurality of crystallization solutions of Crystallization Solution Set II
wherein the Crystallization Solution Set II consists of:
(1) A1 containing 0.3 M ammonium chloride, 0.1 M citric acid, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 4,
(2) A2 containing 0.3 M ammonium formate, 0.1 M potassium nitrate, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 6.6,
(3) B1 containing 0.3 M ammonium nitrate, 0.1 M sodium citrate tribasic dihydrate, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 5.9,
(4) B2 containing 0.3 M ammonium phosphate monobasic, 0.1 M MES monohydrate, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 5.7,
(5) C1 containing 0.3 M ammonium sulfate, 0.1 M BIS-TRIS, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 6.8,
(6) C2 containing 0.3 M di-ammonium tartrate, 0.1 M sodium acetate trihydrate, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 5.8,
(7) D1 containing 0.3 M cadmium chloride, 0.1 M sodium acetate trihydrate, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 3.7,
(8) D2 containing 0.3 M cadmium sulfate, 0.1 M imidazole, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 4.6,
(9) E1 containing 0.3 M caesium chloride, 0.1 M HEPES, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 7.6,
(10) E2 containing 0.3 M calcium acetate, 0.1 M BICINE, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 6.4,
(11) F1 containing 0.3 M calcium chloride, 0.1 M BIS-TRIS propane, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 8.5,
(12) F2 containing 0.3 M cobalt(II) chloride hexahydrate, 0.1 M ammonium citrate, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 3.2,
(13) G1 containing 0.3 M di-ammonium hydrogen citrate, 0.1 M magnesium formate, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 4.9,
(14) G2 containing 0.3 M DL-malic acid, 0.1 M sodium cacodylate trihydrate, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 2.9,
(15) H1 containing 0.3 M iron(III) chloride hexahydrate, 0.1 M potassium nitrate, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 1.1,
(16) H2 containing 0.3 M lithium chloride, 0.1 M potassium formate, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 6.3,
(17) A6 containing 0.3 M lithium sulfate monohydrate, 0.1 M CHES, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 9.5,
(18) A7 containing 0.3 M magnesium acetate tetrahydrate, 0.1 M citric acid, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 4.7,
(19) B6 containing 0.3 M magnesium chloride hexahydrate, 0.1 M sodium cacodylate trihydrate, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 5.8,
(20) B7 containing 0.3 M magnesium nitrate hexahydrate, 0.1 M HEPES, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 7.4,
(21) C6 containing 0.3 M magnesium sulfate heptahydrate, 0.1 M potassium formate, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 6.1,
(22) C7 containing 0.3 M nickel(II) chloride hexahydrate, 0.1 M MES monohydrate, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 5.7,
(23) D6 containing 0.3 M potassium bromide, 0.1 M BIS-TRIS, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 6.6,
(24) D7 containing 0.3 M potassium phosphate monobasic, 0.1 M Tris, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 6.1,
(25) E6 containing 0.3 M potassium sodium tartrate tetrahydrate, 0.1 M BICINE, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 8.8,
(26) E7 containing 0.3 M potassium tartrate, 0.1 M imidazole, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 7.3,
(27) F6 containing 0.3 M potassium thiocyanate, 0.1 M ammonium citrate, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 5.8,
(28) F7 containing 0.3 M sodium bromide, 0.1 M sodium cacodylate trihydrate, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 6.5,
(29) G6 containing 0.3 M sodium chloride, 0.1 M Tris, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 8.1,
(30) G7 containing 0.3 M sodium fluoride, 0.1 M CHES, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 9.6,
(31) H6 containing 0.3 M sodium formate, 0.1 M citric acid, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 4.5,
(32) H7 containing 0.3 M sodium iodide, 0.1 M sodium acetate trihydrate, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 4.7,
(33) A11 containing 0.3 M sodium malonate, 0.1 M sodium citrate tribasic dihydrate, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 6.8,
(34) A12 containing 0.3 M sodium nitrate, 0.1 M MES monohydrate, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 6.1,
(35) B11 containing 0.3 M sodium phosphate monobasic monohydrate, 0.1 M BIS-TRIS, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 5.8,
(36) B12 containing 0.3 M sodium sulfate, 0.1 M BIS-TRIS propane, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 9.3,
(37) C11 containing 0.3 M sodium thiocyanate, 0.1 M HEPES, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 7.8,
(38) C12 containing 0.3 M succinic acid, 0.1 M imidazole, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 3.4,
(39) D11 containing 0.3 M tri-methylamine N-oxide, 0.1 M BICINE, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 8.6,
(40) D12 containing 0.3 M tri-potassium citrate, 0.1 M BIS-TRIS propane, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 9.8,
(41) E11 containing 10% Jeffamine M-600, 0.1 M HEPES, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 7.5,
(42) E12 containing 0.3 M zinc chloride, 0.1 M magnesium formate, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 5.7,
(43) F11 containing 0.3 M zinc sulfate heptahydrate, 0.1 M sodium citrate tribasic dihydrate, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 4,
(44) F12 containing 0.1 M CHES, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 9.9,
(45) G11 containing 0.1 M imidazole, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 6.6,
(46) G12 containing 0.1 M magnesium formate, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 5.9,
(47) H11 containing 0.1 M sodium acetate trihydrate, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 4.7, and
(48) H12 containing 0.1 M Tris, 22.5% PolyPure PEG 0.3-10 kD and having a pH of 8.1.
2. The set of crystallization solutions of claim 1 , wherein the set of crystallization solutions comprises at least 5 of the crystallization solutions of Crystallization Solution Set II.
3. The set of crystallization solutions of claim 1 , wherein the set of crystallization solutions comprises at least 2 of the crystallization solutions of Crystallization Solution Set II with different concentration of polymer.
4. The set of crystallization solutions of claim 1 , wherein the set of crystallization solutions comprises at least 2 of the crystallization solutions of Crystallization Solution Set II with different concentration of salt.
5. The set of crystallization solutions of claim 1 , wherein the set of crystallization solutions comprises at least 2 of the crystallization solutions of Crystallization Solution Set II with different types of salt.
6. The set of crystallization solutions of claim 1 , wherein the set of crystallization solutions comprises at least 2 of the crystallization solutions of Crystallization Solution Set II with different buffers.
7. The set of crystallization solutions of claim 1 , wherein the set of crystallization solutions comprises at least 2 of the crystallization solutions of Crystallization Solution Set II with different concentration of buffers.
8. The set of crystallization solutions of claim 1 , wherein the set of crystallization solutions comprises at least 2 of the crystallization solutions of Crystallization Solution Set II and is suitable for liquid-liquid protein crystallization.
9. A kit comprising at least one crystallization device and a set of crystallization solutions according to claim 1 .