Light absorbance measurement method and apparatus
Methods and apparatuses for measuring a light absorbance are provided. The method measures light absorbance of at least one detection chamber of a microfluidic device, including the detection chamber and at least one reference chamber. The detection chamber may accommodate a test subject. The method includes detecting a plurality of reference transmitted light intensities for the at least one reference chamber and estimating a value between the plurality of reference transmitted light intensities through nonlinear approximation. The estimated value is then applied to light absorbance measurement of the detection chamber to reduce a light absorbance error of the detection chamber.
1. A light absorbance measurement apparatus comprising:
a microfluidic device which comprises at least one detection chamber and at least one reference chamber, the at least one detection chamber accommodating a test subject;
at least one light source which emits light to the at least one reference chamber and the detection chamber;
at least one optical detector corresponding to the at least one light source, the optical detector detecting an intensity of light transmitted through the at least one reference chamber and the detection chamber; and
a controller which calculates a plurality of reference transmitted light intensities for the at least one reference chamber, estimates a value between the plurality of reference transmitted light intensities through nonlinear approximation, and applies the estimated value to light absorbance measurement of the detection chamber.
2. The light absorbance measurement apparatus according to claim 1 , wherein the controller calculates the light absorbance as a ratio between a reference transmitted light intensity of the reference chamber and a detection transmitted light intensity of the at least one detection chamber.
3. The light absorbance measurement apparatus according to claim 1 , wherein the at least one light source includes a plurality of light sources and the at least one optical detector includes a plurality of optical detectors, the plurality of light sources and the plurality of optical detectors being provided at positions corresponding to positions of the at least one reference chamber and the at least one detection chamber, and
wherein the controller performs a control operation to turn on one of the plurality of light sources and to turn off the other light sources when transmitted light intensity detection is performed.
4. The light absorbance measurement apparatus according to claim 3 , wherein the at least one reference chamber includes a first reference chamber and a second reference chamber, and
wherein the controller detects the plurality of reference transmitted light intensities in an order such that a reference transmitted light intensity of the first reference chamber is detected, a reference transmitted light intensity of the second reference chamber is detected, a reference transmitted light intensity of the first reference chamber is again detected; and
wherein the controller estimates a value between the detected reference transmitted light intensities through nonlinear approximation.
5. The light absorbance measurement apparatus according to claim 4 , wherein the first reference chamber, the second reference chamber, and the detection chamber are provided in a concentric arrangement on the microfluidic device.
6. The light absorbance measurement apparatus according to claim 3 , wherein the at least one reference chamber includes a first reference chamber, a second reference chamber, and a third reference chamber, and
wherein the controller detects the plurality of reference transmitted light intensities in an order such that a reference transmitted light intensity of the first reference chamber is detected, a reference transmitted light intensity of the second reference chamber is detected, a reference transmitted light intensity of the third reference chamber is detected, a reference transmitted light intensity of the first reference chamber is again detected, and
wherein the controller estimates a value between the detected reference transmitted light intensities through nonlinear approximation.
7. The light absorbance measurement apparatus according to claim 6 , wherein the first reference chamber, the second reference chamber, and the third reference chamber are provided in a concentric arrangement on the microfluidic device.
8. The light absorbance measurement apparatus according to claim 7 , wherein the first reference chamber, the second reference chamber, and the third reference chamber are provided at about equal intervals along a circumferential direction of the microfluidic device.
9. The light absorbance measurement apparatus according to claim 7 , wherein the third reference chamber, the first reference chamber, and the detection chamber are provided in a concentric arrangement on the microfluidic device.
10. The light absorbance measurement apparatus according to claim 3 , wherein the at least one reference chamber includes only a single reference chamber.
11. The light absorbance measurement apparatus according to claim 10 , wherein the controller detects the plurality of reference transmitted light intensities during a plurality of rotation periods of the microfluidic device.