Probes for detecting the presence of
View Patent ↗Oligonucleotides useful for determining the presence of Trichomonas vaginalis in a test sample.
1. A probe for use in determining the presence of Trichomonas vaginalis in a sample, the probe consisting of an oligonucleotide and one or more labels, each of the labels being joined to the oligonucleotide with a non-nucleotide linker, wherein the oligonucleotide consists of a target-complementary sequence and, optionally, one or more flanking sequences which, under stringent hybridization conditions, do not stably bind to a target nucleic acid derived from Trichomonas vaginalis when the target-complementary sequence is stably hybridized to the target nucleic acid, and wherein the base sequence of the target-complementary sequence consists of the base sequence of SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35 or SEQ ID NO:36, or an RNA/DNA combination equivalent of any of the foregoing.
2. The probe of claim 1 , wherein the probe includes one or more of the flanking sequences.
3. The probe of claim 2 , wherein the probe includes two of the flanking sequences directly joined to opposite ends of the target-complementary sequence.
4. The probe of claim 3 , wherein the two flanking sequences are complementary to each other.
5. The probe of claim 2 , wherein the probe includes two of the flanking sequences joined to opposite ends of the target-complementary sequence, each of the two flanking sequences being directly joined to the target-complementary sequence or joined to the target-complementary sequence by means of a non-nucleotide linker.
6. The probe of claim 5 , wherein the two flanking sequences are complementary to each other.
7. The probe of claim 1 , wherein the probe does not include the flanking sequences.
8. The probe of claim 1 , wherein the target-complementary sequence is an RNA or DNA analog or contains one or more modified nucleosides.
9. The probe of claim 8 , wherein the base sequence of the target-complementary sequence consists of the base sequence of SEQ ID NO:34 or SEQ ID NO:36, and wherein each of the ribonucleosides of the target-complementary sequence has a 2′-O-methyl substitution to the ribofuranosyl moiety.
10. The probe of claim 8 , wherein the base sequence of the target-complementary sequence consists of the base sequence of SEQ ID NO:33 or SEQ ID NO:35, and wherein the target-complementary sequence is a peptide nucleic acid.
11. The probe of claim 1 , wherein the one or more labels are non-isotopic.
12. The probe of claim 11 , wherein the one or more labels include at least one of a chemiluminescent molecule and a fluorescent molecule.
13. The probe of claim 11 , wherein the one or more labels include a pair of interacting labels.
14. The probe of claim 13 , wherein the probe includes two of the flanking sequences joined to opposite ends of the target-complementary sequence, and wherein the flanking sequences are complementary to each other.
15. The probe of claim 14 , wherein the probe is a molecular beacon probe or a molecular torch probe.
16. The probe of claim 1 , wherein the stringent hybridization conditions include a temperature of about 60° C. and a salt concentration of about 1.5M.
17. A method for determining the presence of Trichomonas vaginalis in a sample, the method comprising the steps of:
(a) contacting a sample with the probe of claim 1 under conditions permitting the probe to preferentially hybridize to nucleic acid derived from Trichomonas vaginalis and not to nucleic acid derived from Trichomonas tenax ; and
(b) determining whether a hybrid comprising the probe hybridized to the Trichomonas vaginalis -derived nucleic acid has formed as indication of the presence of Trichomonas vaginalis in the sample.
18. The method of claim 17 , wherein the probe includes two of the flanking sequences joined to opposite ends of the target-complementary sequence, each of the two flanking sequences being directly joined to the target-complementary sequence or joined to the target-complementary sequence by means of a non-nucleotide linker.
19. The method of claim 18 , wherein the two flanking sequences are complementary to each other.
20. The method of claim 17 , wherein the probe does not include the flanking sequences.