Treatment of bone-related cancers using placental stem cells
View Patent ↗Provided herein are methods of suppression of proliferation and growth of cells of bone-related cancers, e.g., multiple myeloma or chondrosarcoma cells, using placental cells, e.g., the placental stem cells described herein, and populations of such placental cells. Also provided herein are methods of treating individuals having cells of a bone-related cancer.
1. An in vitro method of suppressing the proliferation of cells of a bone-related cancer, comprising contacting said cells of a bone-related cancer with a plurality of placental stem cells for a time sufficient for said placental stem cells to suppress proliferation of said cells of a bone-related cancer, as compared to a plurality of said cells of a bone-related cancer not contacted with placental stem cells, wherein said placental stem cells are CD34 − , CD 10 + , CD 105 + and CD200 + as detectable by flow cytometry, and are not trophoblasts, cytotrophoblasts or bone marrow-derived mesenchymal stem cells, and wherein said cells of a bone-related cancer are multiple myeloma cells, chondrosarcoma cells, osteosarcoma cells, Ewing sarcoma cells, chordoma cells, cells of a malignant fibrous histiocytoma of bone, or cells of a fibrosarcoma of bone not prostate cancer cells.
2. The method of claim 1 , wherein said placental cells are CD34 − , CD45 − , CD10 + , CD90 + , CD105 + and CD200 + , as detectable by flow cytometry.
3. The method of claim 1 , wherein said placental cells are CD34 − , CD45 − , CD10 + , CD80 − , CD86 − , CD90 + , CD105 − and CD200 + , as detectable by flow cytometry.
4. The method of claim 1 , wherein said placental cells suppress proliferation of said cells of a bone-related cancer by at least 50% compared to proliferation of an equivalent number of cells of a bone-related cancer in the absence of said placental cells.
5. A method of treating a bone related cancer, comprising administering to a subject having a bone-related cancer a plurality of placental stem cells for a time sufficient for said placental stem cells to suppress proliferation of cells of said bone-related cancer,
wherein said placental stem cells are CD34 − , CD 10 + , CD 105 + and CD200 + as detectable by flow cytometry, and are not trophoblasts, cytotrophoblasts or bone marrow-derived mesenchymal stem cells,
and wherein said cells of a bone-related cancer are multiple myeloma cells, chondrosarcoma cells, osteosarcoma cells, Ewing sarcoma cells, chordoma cells, a malignant fibrous histiocytoma of bone, or a fibrosarcoma of bone.
6. The method of claim 5 , wherein said placental cells are CD34 − , CD45 − , CD10 + , CD 90 + , CD 105 + and CD200 + , as detectable by flow cytometry.
7. The method of claim 5 , wherein said placental cells are CD34 − , CD45 − , CD10 + , CD80 − , CD86 − , CD 90 + , CD 105 + and CD200 + , as detectable by flow cytometry.
8. The method of claim 5 , wherein said subject is human.
9. The method of claim 5 , wherein said placental stem cells are administered at or adjacent to a bone lesion caused by said bone-related cancer.
10. The method of claim 5 , wherein at least 1×10 8 placental stem cells are administered to said individual.