Compositions Produced Using Enteric Pathogens and Methods of Use
The present invention provides compositions including polypeptides having the characteristics of polypeptides expressed by a reference microbe such E. coli or Salmonella . Examples of Salmonella strains that can be used include, for instance, S. enterica serovar Newport, S. enterica serovar Enteritidis, S. enterica serovar Typhimurium, and S. enterica serovar Dublin. Also provided are compositions including polypeptides having a particular molecular weight and a mass fingerprint that includes polypeptide fragments having a particular set of masses, or polypeptides having an amino acid sequence with at least about 95% identity with an amino acid sequence, wherein the polypeptide has seroreactive activity. The present invention also provides methods of making and methods of using such compositions.
1 . A composition comprising:
a first isolated polypeptide having a molecular weight of about 82 kDa as measured following electrophoresis on a sodium dodecyl sulfate (SDS)-polyacrylamide gel, wherein the first isolated polypeptide has a mass fingerprint similar to a mass fingerprint of a polypeptide of about 82 kDa obtained from S. enterica serovar Newport strain MS020508 after growth in low metal conditions;
a second isolated polypeptide having a molecular weight of about 80 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the second isolated polypeptide has a mass fingerprint similar to a mass fingerprint of a polypeptide of about 80 kDa obtained from S. enterica serovar Newport strain MS020508 after growth in low metal conditions; and
a third isolated polypeptide having a molecular weight of about 74 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the third isolated polypeptide has a mass fingerprint similar to a mass fingerprint of a polypeptide of about 74 kDa obtained from S. enterica serovar Newport strain MS020508 after growth in low metal conditions; and
a fourth isolated polypeptide having a molecular weight of about 65 kDa as measured following electrophoresis on an SDS-polyacrylamide gel, wherein the fourth isolated polypeptide has a mass fingerprint similar to a mass fingerprint of a polypeptide of about 65 kDa obtained from S. enterica serovar Newport strain MS020508 after growth in low metal conditions;
2 - 38 . (canceled)
39 . A composition comprising a polypeptide comprising the amino acid sequence of SEQ ID No 1315.
40 . The composition of claim 39 further comprising the amino acid sequence of SEQ ID NO:1318.
41 . The composition of claim 39 further comprising amino acids 3-16 of SEQ ID NO:1328.
42 . The composition of claim 39 further comprising amino acids 13-22 of SEQ ID NO:1325.
43 . The composition of claim 40 further comprising amino acids 3-16 of SEQ ID NO:1328.
44 . The composition of claim 40 further comprising amino acids 13-22 of SEQ ID NO:1325.
45 . The composition of claim 44 further comprising amino acids 3-16 of SEQ ID NO:1328.
46 . The composition of claim 41 further comprising amino acids 13-22 of SEQ ID NO:1325.
47 . The composition of claim 39 wherein the polypeptide is covalently bound to a carrier polypeptide.
48 . A composition comprising the amino acid sequence of SEQ ID NO:1318.
49 . The composition of claim 48 further comprising amino acids 3-16 of SEQ ID NO:1328.
50 . The composition of claim 49 further comprising amino acids 13-22 of SEQ ID NO:1325.
51 . The composition of claim 48 further comprising amino acids 13-22 of SEQ ID NO:1325.
52 . The composition of claim 48 wherein the polypeptide is covalently bound to a carrier polypeptide.
53 . A composition comprising the amino acid sequence of SEQ ID NO:766.
54 . The composition of claim 53 further comprising amino acids 1-8 of SEQ ID NO:769.
55 . The composition of claim 53 wherein the polypeptide is covalently bound to a carrier polypeptide.
56 . A composition comprising the amino acid sequence of SEQ ID NO:776 and amino acids 1-13 of SEQ ID NO:793.
57 . The composition of claim 56 wherein the polypeptide is covalently bound to a carrier polypeptide.