IP Library › Granted Patent US 9,012,189
Granted Patent B2
US 9,012,189 · App. 13/025,805 · Granted Apr 21, 2015

Modified alcohol dehydrogenases for the production of fuels and chemicals

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Quick Facts
Patent No.
US 9,012,189
App. No.
13/025,805
Granted
Apr 21, 2015
Kind
B2
Abstract

The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.

Claims (10)

1. A modified alcohol dehydrogenase (ADH) comprising one or more modifications at positions corresponding to amino acids selected from the group consisting of: (a) tyrosine 50 of the wild-type Lactococcus lactis AdhA (SEQ ID NO: 185); (b) glutamine 77 of the wild-type Lactococcus lactis AdhA (SEQ ID NO: 185); (c) valine 108 of the wild-type Lactococcus lactis AdhA (SEQ ID NO: 185); (d) tyrosine 113 of the wild-type Lactococcus lactis AdhA (SEQ ID NO: 185); (e) isoleucine 212 of the wild-type Lactococcus lactis AdhA (SEQ ID NO: 185); and (f) leucine 264 of the wild-type Lactococcus lactis AdhA (SEQ ID NO: 185), wherein said modified ADH is at least 95% identical to SEQ ID NO: 185 and has ADH activity.

2. The modified alcohol dehydrogenase (ADH) of claim 1 , wherein said tyrosine 50 residue is replaced with a phenylalanine or tryptophan residue.

3. The modified alcohol dehydrogenase (ADH) of claim 1 , wherein said glutamine 77 residue is replaced with an arginine or serine residue.

4. The modified alcohol dehydrogenase (ADH) of claim 1 , wherein said valine 108 residue is replaced with a serine or alanine residue.

5. The modified alcohol dehydrogenase (ADH) of claim 1 , wherein said tyrosine 113 residue is replaced with a phenylalanine or glycine residue.

6. The modified alcohol dehydrogenase (ADI-I) of claim 1 , wherein. said isoleucine 212 residue is replaced with a threonine, alanine, or valine residue.

7. The modified alcohol dehydrogenase (ADH) of claim 1 , wherein said leucine 264 residue is replaced with a valine residue.

8. The modified alcohol dehydrogenase (ADH) of claim 1 , wherein the ADH is codon optimized for expression in a host cell.

9. The modified alcohol dehydrogenase (ADH) of claim 8 , wherein said host cell is yeast.

10. The modified alcohol dehydrogenase (ADH) of claim 8 , wherein said host cell is Escherichia coli.

Assignments (13)
RELEASE OF SECURITY INTEREST Recorded Jan 6, 2021
From: WILMINGTON SAVINGS FUND SOCIETY, FSB
To: GEVO, INC.; GEVO DEVELOPMENT, LLC; AGRI-ENERGY, LLC
Reel/Frame 054912/0709 →
PATENT SECURITY AGREEMENT Recorded Jan 14, 2020
From: GEVO, INC.; GEVO DEVELOPMENT, LLC; AGRI-ENERGY, LLC
To: WILMINGTON SAVINGS FUND SOCIETY, FSB, AS COLLATERAL TRUSTEE
Reel/Frame 051591/0697 →
RELEASE OF SECURITY INTEREST Recorded Jan 13, 2020
From: WILMINGTON SAVINGS FUND SOCIETY, FSB, AS COLLATERAL TRUSTEE
To: GEVO, INC.; AGRI-ENERGY, LLC; GEVO DEVELOPMENT, LLC
Reel/Frame 051497/0560 →
RELEASE OF SECURITY INTEREST Recorded Jun 21, 2017
From: WILMINGTON SAVINGS FUND SOCIETY, FSB, AS COLLATERAL TRUSTEE
To: GEVO, INC.; GEVO DEVELOPMENT, LLC; AGRI-ENERGY, LLC
Reel/Frame 042940/0081 →
PATENT SECURITY AGREEMENT Recorded Jun 21, 2017
From: GEVO, INC.; GEVO DEVELOPMENT, LLC; AGRI-ENERGY, LLC
To: WILMINGTON SAVINGS FUND SOCIETY, FSB, AS COLLATERAL TRUSTEE
Reel/Frame 042940/0103 →
RELEASE OF SECURITY INTEREST Recorded Oct 4, 2016
From: TRIPLEPOINT CAPITAL LLC
To: GEVO, INC.
Reel/Frame 040219/0177 →
RELEASE OF SECURITY INTEREST Recorded Jan 23, 2015
From: WB GEVO, LTD., AS SUCCESSOR IN INTEREST TO WHITEBOX ADVISORS LLC, AS ADMINISTRATIVE AGENT
To: GEVO, INC.; GEVO DEVELOPMENT, LLC; AGRI-ENERGY, LLC
Reel/Frame 034806/0926 →
PATENT SECURITY AGREEMENT Recorded Jun 10, 2014
From: GEVO, INC.; GEVO DEVELOPMENT, LLC; AGRI-ENERGY, LLC
To: WILMINGTON SAVINGS FUND SOCIETY, FSB, AS COLLATERAL TRUSTEE
Reel/Frame 033120/0453 →
AMENDED AND RESTATED PATENT SECURITY AGREEMENT Recorded Jun 6, 2014
From: GEVO, INC.; GEVO DEVELOPMENT, LLC; AGRI-ENERGY, LLC
To: WB GEVO, LTD., AS ADMINISTRATIVE AGENT
Reel/Frame 033101/0302 →
PATENT SECURITY AGREEMENT Recorded May 13, 2014
From: GEVO, INC.; GEVO DEVELOPMENT, LLC; AGRI-ENERGY, LLC
To: WHITEBOX ADVISORS LLC, AS ADMINISTRATIVE AGENT
Reel/Frame 032891/0881 →
SECURITY AGREEMENT Recorded Jan 23, 2014
From: GEVO, INC. (AS GRANTOR)
To: TRIPLEPOINT CAPITAL LLC (AS GRANTEE)
Reel/Frame 032123/0844 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 1, 2011
From: MEINHOLD, PETER
To: GEVO, INC.
Reel/Frame 026063/0683 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 1, 2011
From: BASTIAN, SABINE; ARNOLD, FRANCES
To: THE CALIFORNIA INSTITUTE OF TECHNOLOGY
Reel/Frame 026063/0779 →