Purification of biologically-produced 1,3-propanediol
View Patent ↗A process for purifying 1,3-propanediol from the fermentation broth of a cultured E. coli that has been bioengineered to synthesize 1,3-propanediol from sugar is provided. The basic process entails filtration, ion exchange and distillation of the fermentation broth product stream, preferably including chemical reduction of the product during the distillation procedure. Also provided are highly purified compositions of 1,3-propanediol.
1. A process of purifying biologically-produced 1,3-propanediol from the fermentation broth of an organism able to produce 1,3-propanediol, comprising the steps of:
(a) subjecting the fermentation broth to filtration;
(b) subjecting the product of step (a) to ion exchange purification wherein anionic and cationic molecules are removed;
(c) subjecting the product of step (b) to chemical reduction; and
(d) subjecting the product of step (c) to a distillation procedure comprising at least two distillation columns wherein one of said distillation columns removes molecules having a boiling point exceeding the boiling point of 1,3-propanediol and the other of the distillation columns removes molecules having a boiling point below the boiling point of 1,3-propanediol.
2. The process of claim 1 , wherein said chemical reduction is selected from the group consisting of hydrogenation and hydroboration.
3. The process of claim 2 , wherein said chemical reduction is hydrogenation.
4. The process of claim 1 , further comprising wherein the fermentation broth is subjected to an evaporation step.
5. The process of claim 4 , wherein said evaporation step is conducted during step (b).
6. The process of claim 1 , wherein the filtration process of step (a) comprises more than one separate filtration procedure.
7. The process of claim 6 , wherein the filtration process of step (a) comprises a first and a second filtration procedure, said first filtration procedure comprising subjecting the fermentation broth to microfiltration comprising removing molecules having a size greater than 0.2 microns and said second filtration procedure comprising removing molecules having a molecular weight greater than about 200 Daltons.
8. The process of claim 6 , wherein the filtration process of step (a) comprises a first, a second and a third filtration procedure: said first filtration procedure comprising subjecting the fermentation broth to microfiltration comprising removing molecules having a size greater than 0.2 microns; said second filtration procedure comprising subjecting the fermentation broth to ultrafiltration comprising removing molecules having a molecular weight greater than about 5000 Daltons, and said third filtration procedure comprising subjecting the fermentation broth to nanofiltration comprising removing molecules having a molecular weight greater than about 200 to 400 Daltons.
9. The process of claim 1 , wherein the ion exchange process of step (b) comprises more than one separate ion exchange procedure.
10. The process of claim 9 , wherein the ion exchange process of step (b) comprises subjecting the product of step (a) to one or more series of ion exchange procedures wherein each series of procedures comprises exposing the product to a strong acid cation exchange resin followed by exposure to a weak base anion exchange resin.
11. The process of claim 9 , wherein the ion exchange process of step (b) comprises subjecting the product of step (a) to two series of ion exchange procedures wherein each series comprises exposing the product to a strong acid cation exchange resin followed by exposing the product to a weak base anion exchange resin.
12. The process of claim 11 , wherein the ion exchange process of step (b) further comprises subjecting the product to a mixed ion exchange resin composition comprising a mixture of strong acid cation exchange resin and strong base anion exchange resin.
13. The process of claim of 1 , wherein step (b) comprises subjecting the product of step (a) to two series of ion exchange procedures wherein each series comprises exposing the product to a strong acid cation exchange resin followed by exposing the product to a weak base anion exchange resin; followed by an evaporation step; followed by subjecting the product to a mixed ion exchange resin composition comprising a mixture of strong acid cation exchange resin and strong base anion exchange resin.