Methods of making enhanced, autologous fat grafts
View Patent ↗Cells present in processed lipoaspirate tissue are used to treat patients. Methods of treating patients include processing adipose tissue to deliver a concentrated amount of stem cells obtained from the adipose tissue to a patient. The methods may be practiced in a closed system so that the stem cells are not exposed to an external environment prior to being administered to a patient. Compositions that are administered to a patient include a mixture of adipose tissue and stem cells so that the composition has a higher concentration of stem cells than when the adipose tissue was removed from the patient.
1. A method of making an enhanced, autologous fat graft comprising extracting from a subject a first portion of adipose tissue to be used as a fat graft then supplementing said fat graft with a population of concentrated, disaggregated, adipose-derived stem cells and endothelial precursor cells in a buffered solution, wherein said stem cells and endothelial precursor cells are prepared by disaggregating and concentrating the stem cells and endothelial precursor cells from a second portion of extracted adipose tissue from said subject, so as to make said enhanced, autologous fat graft.
2. The method of claim 1 , wherein said disaggregated adipose-derived stem cells and endothelial precursor cells are prepared by:
(a) disaggregating said second portion of extracted adipose tissue to generate a cell suspension; and
(b) separating a population of adipose-derived cells that comprises stem cells and endothelial precursor cells from the cell suspension by buoyant density sedimentation, filtration, or centrifugation.
3. The method of claim 2 , wherein the second portion of extracted adipose tissue is disaggregated in (a) by enzymatic digestion or mechanical force.
4. The method of claim 2 , further comprising reducing the presence of mature adipocytes in the cell suspension generated at step (a) prior to step (b).
5. The method of claim 2 , wherein the population of adipose-derived cells that comprises stem cells and endothelial precursor cells in (b) is separated by centrifugation.
6. The method of claim 2 , wherein the second portion of extracted adipose tissue is disaggregated in (a) by enzymatic digestion.
7. The method of claim 2 , wherein said population of concentrated, disaggregated, adipose-derived stem cells and endothelial precursor cells is prepared in a device that operates in a closed system.
8. The method of claim 1 , wherein said first portion of extracted adipose tissue is supplemented with said population of concentrated, disaggregated adipose-derived stem cells and endothelial precursor cells in a device that operates in a closed system.
9. The method of claim 1 , wherein said population of concentrated, disaggregated adipose-derived stem cells and endothelial precursor cells is not cultured.
10. A method of making an enhanced, autologous fat graft comprising:
(a) disaggregating a first portion of extracted adipose tissue from a subject with an enzyme or mechanical force to generate a cell suspension;
(b) concentrating the cells in said cell suspension by buoyant density sedimentation, filtration or centrifugation to obtain a population of concentrated, disaggregated adipose-derived stem cells and endothelial precursor cells in a buffered solution; and
(c) supplementing a second portion of extracted adipose tissue to be used as a fat graft from said subject with the population of concentrated, disaggregated adipose-derived stem cells and endothelial precursor cells in the buffered solution, so as to make said enhanced, autologous fat graft.
11. The method of claim 10 , wherein the population of concentrated, disaggregated adipose-derived stem cells and endothelial precursor cells in (b) is prepared by centrifugation.
12. The method of claim 10 , wherein said population of concentrated, disaggregated adipose-derived stem cells and endothelial precursor cells is prepared in a device that operates in a closed system.
13. The method of claim 10 , wherein said second portion of extracted adipose tissue is supplemented with said population of concentrated, disaggregated adipose-derived stem cells and endothelial precursor cells in a device that operates in a closed system.
14. The method of claim 10 , further comprising reducing the presence of mature adipocytes in the cell suspension generated at step (a) prior to step (b).
15. The method of claim 10 , wherein said population of concentrated, disaggregated adipose-derived stem cells and endothelial precursor cells is not cultured.
16. A method of making an enhanced, autologous fat graft comprising extracting from a subject a first portion of adipose tissue to be used as a fat graft then supplementing said fat graft with a population of concentrated, disaggregated adipose-derived stem cells in a buffered solution, wherein said stem cells are prepared by disaggregating and concentrating the stem cells from a second portion of extracted adipose tissue from said subject, so as to make said enhanced, autologous fat graft.
17. The method of claim 16 , wherein said population of concentrated, disaggregated adipose-derived stem cells is not cultured.
18. The method of claim 16 , wherein said population of concentrated, disaggregated adipose-derived stem cells is prepared in a device that operates in a closed system.
19. The method of claim 16 , wherein said second portion of extracted adipose tissue is supplemented with said population of concentrated, disaggregated stem cells in a device that operates in a closed system.
20. The method of claim 1 , wherein the enhanced, autologous fat graft further comprises an additive.
21. The method of claim 10 , wherein the enhanced, autologous fat graft further comprises an additive.
22. The method of claim 16 , wherein the enhanced, autologous fat graft further comprises an additive.