Antimicrobial wash and carrier solutions and uses thereof
View Patent ↗Novel wash and carrier solutions ideally suited for topical administration (e.g., as wash solutions and as carrier solutions). The solutions described herein are non-toxic, non-irritating, isotonic, possess non-specific broad-spectrum antimicrobial properties, and have an alkaline pH. Also disclosed are sterile preparations of the solutions and methods of using the same.
1. A method of washing a wound comprising washing the wound with an aqueous antimicrobial and preservative alkaline solution, said solution comprising:
(a) a buffer system comprising a Tris buffer system for maintaining the solution at a pH in a range of about 8 to about 9.5;
(b) a non-ionic surfactant system comprising at least one surfactant having a multiplicity of ether linkages;
(c) metal ion chelating agent comprising EDTA or its pharmaceutically acceptable salts; and
(d) a non-ionic preservative having anti-microbial activity comprising benzyl alcohol wherein the concentration of benzyl alcohol is about 0.5% (w/w) to about 2.0% (w/w),
wherein the solution has a pH in the range of about 8 to about 9.5 and has anti-microbial properties, and the aqueous antimicrobial and preservative alkaline solution is non-irritating.
2. The method of claim 1 , wherein the buffer system is a Tris buffer system comprising tromethamine base at a concentration of about 0.3 to about 0.55% (w/w) and tromethamine hydrochloride at a concentration of about 0.3 to about 0.45% (w/w).
3. The method of claim 1 , wherein the surfactant is selected from the group consisting of nonoxynol 12, PPG-12/PEG-50 lanolin, phospholipid CDM, phospholipid EFA, phospholipid PTA, PEG-100 stearate, and any other nonoxynol, octoxynol surfactants with different PEG chain lengths.
4. The method of claim 1 , wherein the metal ion chelating agent is selected from the group consisting of disodium EDTA, tetrasodium EDTA, lauroyl EDTA, dilauroyl EDTA and edetate calcium disodium.
5. A method of treating a topical microbial infection of a tissue comprising washing the tissue with an aqueous antimicrobial and preservative alkaline solution, said solution comprising:
(a) a buffer system comprising a Tris buffer system for maintaining the solution at a pH in a range of about 8 to about 9.5;
(b) a non-ionic surfactant system comprising at least one surfactant having a multiplicity of ether linkages;
(c) metal ion chelating agent comprising EDTA or its pharmaceutically acceptable salts; and
(d) a non-ionic preservative having anti-microbial activity comprising benzyl alcohol wherein the concentration of benzyl alcohol is about 0.5% (w/w) to about 2.0% (w/w),
wherein the solution has a pH in the range of about 8 to about 9.5 and has anti-microbial properties, and the aqueous antimicrobial and preservative alkaline solution is non-irritating.
6. A method of preparing a tissue for surgery comprising washing the tissue prior to the surgery with an aqueous antimicrobial and preservative alkaline solution, said solution comprising:
(a) a buffer system comprising a Tris buffer system for maintaining the solution at a pH in a range of about 8 to about 9.5;
(b) a non-ionic surfactant system comprising at least one surfactant having a multiplicity of ether linkages;
(c) metal ion chelating agent comprising EDTA or its pharmaceutically acceptable salts; and
(d) a non-ionic preservative having anti-microbial activity comprising benzyl alcohol wherein the concentration of benzyl alcohol is about 0.5% (w/w) to about 2.0% (w/w),
wherein the solution has a pH in the range of about 8 to about 9.5 and has anti-microbial properties, and the aqueous antimicrobial and preservative alkaline solution is non-irritating.
7. A method of topically delivering a pharmaceutical agent to a tissue comprising:
(a) mixing the pharmaceutical with an aqueous antimicrobial and preservative alkaline solution, said solution comprising:
(i) a buffer system comprising a Tris buffer system for maintaining the solution at a pH in a range of about 8 to about 9.5;
(ii) a non-ionic surfactant system comprising at least one surfactant having a multiplicity of ether linkages;
(iii) metal ion chelating agent comprising EDTA or its pharmaceutically acceptable salts; and
(iv) a non-ionic preservative having anti-microbial activity comprising benzyl alcohol wherein the concentration of benzyl alcohol is about 0.5% (w/w) to about 2.0% (w/w),
wherein the solution has a pH in the range of about 8 to about 9.5 and has anti-microbial properties, and the aqueous antimicrobial and preservative alkaline solution is non-irritating; and
(b) applying the resulting mixture to the tissue.
8. A method of washing a wound comprising washing the wound with an aqueous antimicrobial and preservative alkaline solution, said solution comprising:
(a) a buffer system comprising a Tris buffer system for maintaining the solution at a pH in a range of about 8.2 to about 8.8;
(b) a surfactant system comprising at least one surfactant having a multiplicity of ether linkages;
(c) metal ion chelating agent comprising EDTA or its pharmaceutically acceptable salts at a concentration of about 0.05 wt. % to about 0.15 wt. %; and
(d) a non-ionic preservative having anti-microbial activity comprising benzyl alcohol at a concentration of about 0.5% (w/w) to about 2% (w/w),
wherein the solution has a pH in the range of about 8.2 to about 8.8 and has anti-microbial properties, and the aqueous antimicrobial and preservative alkaline solution is non-irritating.