IP Library Patent Application 13052480
Patent Application
App. No. 13/052,480

CENTRIFUGE CONTAINER

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Quick Facts
Patent No.
US None
App. No.
13/052,480
Abstract

A cell suspension that contains a cell group from which unwanted components have been removed by efficiently washing the cell group is recovered. A centrifuge container ( 1 ) includes a cylindrical container main body ( 2 ) that accommodates a cell suspension and is rotated with a bottom portion ( 2 a ) being directed radially outward; a supernatant suction tube ( 4 ) that has a first opening ( 4 a ) at a position in the depth direction of the container main body ( 2 ) and that suctions a supernatant obtained by centrifuging the cell suspension, in the radial direction of the container main body ( 2 ); and a washing-fluid discharge tube ( 3 ) that have a second opening ( 3 a ) at a position in the depth direction of the container main body ( 2 ) and that discharge a washing fluid in the axial direction toward the bottom portion ( 2 a ) of the container main body ( 2 ).

Claims (8)

1 . A centrifuge container comprising:

a cylindrical container main body that accommodates a cell suspension and is rotated with a bottom portion being directed radially outward;

a supernatant suction tube that has a first opening at a position in the depth direction of the container main body and that suctions a supernatant obtained by centrifuging the cell suspension, from the first opening, in the radial direction of the container main body; and

a washing-fluid discharge tube that has a second opening at a position in the depth direction of the container main body and that discharges a washing fluid from the second opening, in the axial direction toward the bottom portion of the container main body.

2 . A centrifuge container according to claim 1 , wherein the supernatant suction tube and the washing-fluid discharge tube are provided with a common path through which the supernatant and the washing fluid flow, and a valve that is provided at the end of the common path, that opens the second opening when the washing fluid is discharged, and that closes the second opening when the supernatant is suctioned.

3 . A centrifuge container according to claim 1 , further comprising a cell suction tube that has a third opening close to the bottom portion of the container main body and that suctions, from the third opening, a cell suspension that contains a cell group centrifuged in the bottom portion.

4 . A centrifuge container according to claim 1 , further comprising a suspension supply tube for supplying, to the container main body, a cell suspension that contains a cell group to be centrifuged.

5 . A centrifuge container according to claim 4 , wherein the suspension supply tube is formed of piping common to the washing-fluid discharge tube.

Assignments (4)
RELEASE OF SECURITY INTEREST Recorded Jul 3, 2013
From: GENERAL ELECTRIC CAPITAL CORPORATION
To: CYTORI THERAPEUTICS, INC.
Reel/Frame 030734/0778 →
SECURITY AGREEMENT Recorded Dec 3, 2012
From: CYTORI THERAPEUTICS INC.
To: GENERAL ELECTRIC CAPITAL CORPORATION
Reel/Frame 029395/0660 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jun 1, 2011
From: KINOSHITA, AKI
To: OLYMPUS CORPORATION
Reel/Frame 026373/0132 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jun 1, 2011
From: ARM, DOUGLAS M.; SHANAHAN, ROBERT K.; FORNACE, LUCAS V.
To: CYTORI THERAPEUTICS, INC.
Reel/Frame 026373/0172 →