Methods for plant fiber characterization and identification
View Patent ↗Methods are provided for the isolation of biological macromolecules including peptides, proteins, and nucleic acids from mature plant fibers such as cotton fibers and from textiles. The biological macromolecules can be used to characterize the plant fiber (source, origin, genomic composition of producing plant etc.).
1. A method for identifying a processed cotton fiber comprising the steps of
a. isolating DNA from said cotton fiber said DNA being naturally occurring in said cotton fiber; and
b. subjecting said DNA to a detection assay specific for said cotton fiber
wherein said processed cotton fiber is a cotton fiber which has been subjected to at least one of the process steps selected from the group of ginning, baling, knitting, weaving, scouring, desizing, bleaching, mercerizing, dyeing, waxing, carding, spinning and sizing.
2. The method of claim 1 , wherein said detection assay is a polymerase chain reaction based detection assay.
3. The method of claim 1 , wherein said detection assay is a nucleic acid hybridization based detection assay.
4. The method of claim 1 , wherein said detection assay detects the presence or absence of a chimeric gene present in the genome of the cotton plant producing the fibers.
5. The method of claim 4 , wherein said chimeric gene comprises a coding region selected from N-acetylglucosamine transferase, phosphinotricinacetyltransferase, EPSPS, hydroxyphenylpyruvate-dioxygenase, an insecticidal portion of Bacillus thuringiensis crystal protein, poly(ADP-ribose) polymerase, poly(ADP-ribose) glucohydrolase, sucrose synthase, sucrose phosphate synthase, glucanase, cellulose synthase, chitinase, expansin, callose synthase, kinase, nicotinamidase, nicotinate phosphoribosyltransferase, nicotinic acid mononucleotide adenyl transferase, nicotinamide adenine dinucleotide synthetase or nicotine amide phosphorybosyltransferase.
6. The method of claim 4 , wherein said detection assay is an event-specific detection assay.
7. The method of claim 1 , wherein said detection assay detects the presence or absence of specific alleles present in the genome of the cotton plant producing the fibers.
8. The method of claim 1 , wherein said fiber is present in yarns, fabric, or finished apparel.
9. A method for analyzing the genome of a fiber producing cotton plant comprising the steps of
a. isolating DNA from processed fibers of said cotton plant, said DNA being naturally occurring in said cotton fiber; and
b. subjecting said DNA to a genome analysis protocol
wherein said processed cotton fiber is a cotton fiber which has been subjected to at least one of the process steps selected from the group of ginning, baling, knitting, weaving, scouring, desizing, bleaching, mercerizing, dyeing, waxing, carding, spinning and sizing.
10. The method according to claim 9 , wherein said isolated DNA is subjected to a whole genome amplification step prior to subjecting said DNA to a genome analysis protocol.
11. The method according to claim 9 , wherein said method is applied to yarns, fabrics or finished apparel.
12. A method for isolating naturally occurring DNA from processed cotton fibers comprising the steps of
a. removing leaf and stem trash material from the cotton fibers; and
b. incubating said cotton fibers in a lysis buffer containing detergents, proteases and salts for a prolonged time; and
c. processing said lysis buffer according to standard DNA isolation methods and isolating said naturally occurring DNA
wherein said processed cotton fiber is a cotton fiber which has been subjected to at least one of the process steps selected from the group of ginning, baling, knitting, weaving, scouring, desizing, bleaching, mercerizing, dyeing, waxing, carding, spinning and sizing.
13. A method for isolating naturally occurring DNA from woven or knitted fabric comprising processed cotton fibers, said method comprising the steps of
a. unweaving the threads of said fabric;
b. incubating said threads in a lysis buffer containing detergents, proteases and salts for a prolonged time; and
c. processing said lysis buffer according to standard DNA isolation methods and isolating said naturally occurring DNA;
wherein said processed cotton fiber is a cotton fiber which has been subjected to at least one of the process steps selected from the group consisting of ginning, baling, knitting, weaving, scouring, desizing, bleaching, mercerizing, dyeing, waxing, carding, spinning and sizing.
14. A method to determine the relative amounts of different cotton fibers in a mixture of processed cotton fibers comprising the steps of
a. isolating from said mixture of cotton fibers DNA naturally occurring in said cotton fibers;
b. subjecting said DNA to detection assays specific for each of said cotton fibers; and
c. determining the relative amount of each of the cotton fibers
wherein said processed cotton fiber is a cotton fiber which has been subjected to at least one of the process steps selected from the group consisting of ginning, baling, knitting, weaving, scouring, desizing, bleaching, mercerizing, dyeing, waxing, carding, spinning and sizing.
15. A method to certify the identity of traded cotton fibers, said method comprising:
a. recording purchase of certified cotton seeds by registered growers, said cotton seed comprising a specific genome composition and producing a particular brand of cotton fibers;
b. registering bales of raw cotton fibers produced by said registered growers from said certified cotton seed as said particular brand of cotton fibers;
c. authenticating said bales by cross checking with said seed purchase records;
d. providing said registered bales to mills to produce yarns, fabrics or apparel from said brand of cotton fibers;
e. auditing the identity of said brand of cotton fibers at one or more steps using a method for identifying a processed cotton fiber comprising the steps of
1. isolating DNA from said cotton fiber, said DNA being naturally occurring in said cotton fiber; and
2. subjecting said DNA to a detection assay specific for said cotton fiber;
wherein said processed cotton fiber is a cotton fiber which has been subjected to at least one of the process steps selected from the group of ginning, baling, knitting, weaving, scouring, desizing, bleaching, mercerizing, dyeing, waxing, carding, spinning and sizing.
16. The method of claim 12 , wherein said prolonged time is a period of 4 to 100 hours.
17. The method of claim 13 , wherein said prolonged time is a period of 4 to 100 hours.
18. The method of claim 15 , wherein said registered bales are provided to mills to produce yarns, fabrics or apparel predominantly from said brand of cotton fibers.
19. The method of claim 15 , wherein said registered bales are provided to mills to produce yarns, fabrics or apparel exclusively from said brand of cotton fibers.