ALCOHOL PRODUCTION PROCESS
The invention relates to the microbial fermentation of gaseous substrates, particularly to methods of mitigating and/or reducing alcohol toxicity effects on a microbial culture at elevated alcohol concentrations during fermentation. The invention relates particularly to microbial fermentation of substrates comprising CO and the effects of alcohol toxicity are reduced or mitigated by maintaining the temperature below the optimum operating temperature by cooling the fermentation broth.
1 - 20 . (canceled)
21 . A method of reducing alcohol toxicity effects on a microbial culture in an alcohol production fermentation, said method comprising:
identifying an optimum operating temperature or range of the microbial culture
maintaining the microbial culture at a temperature lower than the optimum operating temperature.
22 . The method of claim 21 , wherein the fermentation converts a substrate that includes CO into one or more products, said products including one or more alcohols.
23 . The method of claim 22 , wherein the alcohol is ethanol.
24 . The method of claim 21 , wherein maintaining the microbial culture comprises maintaining the microbial culture at a temperature at least 1° C. lower than the optimum operating temperature of the microbial culture.
25 . The method of claim 21 , wherein the microbial culture comprises one or more carboxydotrophic microorganisms.
26 . The method of claim 25 , wherein the carboxydotrophic microorganisms are alcohol producing acetogens.
27 . The method of claim 26 , wherein the alcohol producing acetogens are selected from the group consisting of Clostridium autoethanogenum, Clostridium ragsdalei , and Clostridium Ljungdahlii.
28 . A method of increasing alcohol concentration in a fermentation broth above a predetermined threshold concentration, said method comprising:
maintaining a fermentation operating temperature at or about an optimum operating temperature of a microbial culture in a fermentation broth, such that the microbial culture converts a substrate to one or more products including one or more alcohols until the concentration of alcohol in the fermentation exceeds the predetermined threshold concentration; then
maintaining the fermentation operating temperature below the optimum operating temperature of the microbial culture.
29 . The method of claim 28 , wherein the substrate comprises CO.
30 . The method of claim 28 , wherein the alcohol is ethanol.
31 . The method of claim 28 , wherein maintaining the fermentation operating temperature below the optimum operating temperature of the microbial culture comprises maintaining the fermentation operating temperature at least 1° C. less than the optimum operating temperature.
32 . The method of claim 28 , wherein the predetermined threshold concentration is at least 40 grams of ethanol per liter of fermentation broth.
33 . The method of claim 28 , wherein the microbial culture comprises one or more carboxydotrophic microorganisms.
34 . The method of claim 33 , wherein the carboxydotrophic microorganisms are alcohol producing acetogens.
35 . The method of claim 34 , wherein the alcohol producing acetogens are selected from the group consisting of Clostridium autoethanogenum, Clostridium ragsdalei , and Clostridium Ljungdahlii.
36 . A microbial fermentation system configured to, in use, reduce alcohol toxicity effects on a microbial culture, said system comprising:
a bioreactor, configured to, in use, contain a fermentation broth;
means for determining alcohol concentration of the fermentation broth;
means for regulating temperature of the fermentation broth.
37 . The system of claim 36 , wherein the means for regulating temperature and the means for determining alcohol concentration are operatively linked such that, in use, as alcohol concentration increases above a predetermined threshold value in a fermentation broth, the means for regulating temperature reduces the temperature of the fermentation broth.