Gene expression profiling in primary ovarian serous papillary tumors and normal ovarian epithelium
View Patent ↗Provided herein are methods for treating ovarian serous papillary carcinoma by delivering Clostridium perfringens enterotoxins to ovarian tumor cells overexpressing claudin 3 or claudin 4 protein or by inhibiting the expression and function of tumor-associated calcium signal transducer 1 (TROP-1/Ep-CAM) gene or gene product. Also provided is a method of detecting ovarian serous papillary carcinoma by examining a tumor sample from the individual for gene or gene product expression levels within a group of genes and performing statistical analysis to determine those genes or gene products which are down-regulated compared to those in a normal individual.
1. A method of detecting ovarian serous papillary carcinoma in an individual, comprising the steps of:
examining a tumor sample from said individual by the expression level of a group of genes consisting of PEG3, MYH11, ECM2, C7, TCF21, TGFBR3, SPARCL1, ALDH1A1, TM4SF3, ABCA8, RNASE4, ITM2A, NR1H4, PLA2G2A, APOD, CHL1, SEPP1, IGF1, SEMACAP3, GPM6A, EBAF, GSTM5, COL14A1, VWF, AOX1, MAF, PIPPIN, NR4A1, COL15A1, SFRP4, MFAP4, PDGFRA, GATM, STAR, LAMA2, FABP4, WISP2, CPE, LRRC17, FMOD, CILP, ITPR1, FGF7, CXCL12, ERG, CLECSF2, VLDLR, NTRK2, PDEIA, NY-REN-7, TENC1, HFL1, GASP, PROS1, PTGIS, ARHI, FLJ32389, DKFZP586A0522, EFEMP1, PTPRD, ABCA6, RPIB9, CPZ, RECK, LOC284244, GEM, HSD11B1, PMP22, GREB1, NID, FLJ36166, PRKAR2B, COX7A1, SDC2, DSIPI, PLA2G5, SMARCA2, PRSS11, SERPINF1, SERPINA3, CXCL12, D8S2298E, MAOB, FLRT2, DPYD, MAP3K5, ANGPTL2, MAPK10, TRPC1, HLF, DSCR1L1, FOSB, IGKC, CDKN1C, PDGFRB, SCRG1, EDNRA, DMD, PON3, FXYD1, PLCL1, DOC1, PSPHL, LMOD1, PECAM1, FLJ31737, BMP6, CG018, FBLN5, FHL1, TNXB, PBX3, PLCL2, TLR5, GAS1, SGCE, EMILIN1, GNG11, MAPRE2, HMOX1, APOA1, C1R, FBN1, MEF2C, TM4SF10, AOC3, TNA, RHOBTB1, SPG20, COL16A1, CHN2, ZFHX1B, CDH11, C1S, PPP1R12B, HOP, ZNF288, F10, GPRK5, and DAB2; and
performing hierarchical cluster analysis on the expression levels of said genes as compared to those in an individual that does not have ovarian serous papillary carcinoma, wherein down-regulation of said genes indicates that said individual has ovarian serous papillary carcinoma.
2. The method of claim 1 , wherein there is at least a 5-fold down-regulation of said genes.
3. The method of claim 1 , wherein said gene expression level is examined by DNA microarray.