Automated cellular material preparation
View Patent ↗Provided are cartridges and systems for effecting automated extraction, isolation, and purification of cellular components—such as nucleic acids—from a cellular sample in assay-ready form. Also provided are related methods of effecting such sample processing.
1. A processing system configured to concentrate and isolate cellular components, comprising:
a plurality of cartridge receivers, each cartridge receiver having a recess configured to receive a purification cartridge, the purification cartridge including a sample vessel, an elution port, and a capture chamber, the capture chamber being slidably affixed relative to the sample vessel and elution port, the capture chamber defining a void in which a capture material is disposed;
a motor configured to actuate the capture chamber from a load position, in which the capture material is in fluid communication with the sample vessel, to an elution position, in which the capture material is in fluid communication with an elution port;
a controller configured to control the motor; and
a tube extending from the cartridge receiver that, when the capture chamber is in the load position, is in fluid communication with the sample vessel and capture material.
2. The system of claim 1 , wherein the controller is capable of modulating processing of two different samples.
3. The system of claim 2 , wherein the controller is capable of modulating simultaneous processing of two or more different samples.
4. The system of claim 2 , wherein the controller is capable of modulating sequential processing of two or more different samples.
5. The processing system of claim 1 , further comprising a thermal element configured to contact at least a portion of the purification cartridge.
6. The processing system of claim 5 , further comprising a shield disposed about the thermal element.
7. The processing system of 1 further comprising at least one magnet configured to secure the purification cartridge in one of the plurality of cartridge receivers.
8. The processing system of 1 further comprising at least one spring loaded grip configured to secure the purification cartridge in one of the plurality of cartridge receivers.
9. The processing system of 1 wherein the controller is further configured to control the system so as to process two different samples at one time.
10. A processing system, comprising:
a plurality of purification cartridges, each cartridge including a sample vessel, an elution port, and a capture chamber, the capture chamber being slidably affixed relative to the sample vessel and elution port, the capture chamber defining a void in which a capture material is disposed;
a plurality of cartridge receivers, each receiver defining a slot in which one of the plurality of purification cartridges is disposed;
a motor configured to actuate the capture chamber from a load position, in which the capture material is in fluid communication with the sample vessel, to an elution position, in which the capture material is in fluid communication with an elution port;
a controller configured to control the motor; and
a tube extending from the cartridge receiver that, when the capture chamber is in the load position, is in fluid communication with the sample vessel and capture material.
11. The fluid processing system of claim 10 , wherein the controller is capable of effecting different processing steps for two or more of two or more samples disposed in two or more purification cartridges disposed in two or more cartridge receivers.
12. The processing system of claim 10 wherein the controller is further configured to control the system so as to effect processing of two or more different samples disposed in two or more purification cartridges disposed in two or more cartridge receivers.
13. The processing system of claim 10 , further comprising at least one thermal element configured to contact at least a portion of at least one of the plurality of purification cartridges.
14. The processing system of 10 further comprising at least one magnet configured to secure at least one of the plurality of purification cartridges in one of the plurality of cartridge receivers.
15. The processing system of 10 further comprising at least one spring loaded grip configured to secure at least one of the plurality of purification cartridges in one of the plurality of cartridge receivers.
16. The processing system of 10 wherein the capture material includes at least one of a microarrays, a porous polymer monolith, a silica membrane, a cellulose packing material, a polycarbonate membranes, and a sequencing flow cell.
17. The processing system of claim 10 , wherein the purification cartridge includes a magnet configured to mix a sample in the sample vessel.
18. A processing system, comprising:
a plurality of cartridge receivers, each receiver having a recess configured to hold at least part of a purification cartridge, the purification cartridge including a sample vessel, an elution port, and a capture chamber, the capture chamber being slidably affixed relative to the sample vessel and elution port, the capture chamber defining a void in which a capture material is disposed;
at least one of the plurality of cartridge receivers including a thermal element configured to heat a molecular capture region on the purification cartridge to above about 40° C., or to cool the molecular capture region to below about 10° C., or both; and
a motor configured to actuate the capture chamber from a load position, in which the capture material is in fluid communication with the sample vessel, to an elution position, in which the capture material is in fluid communication with an elution port; and
a controller configured to control the motor.
19. The processing system of claim 18 , further comprising a shield disposed about the thermal element.
20. The processing system of 18 further comprising at least one magnet configured to secure the purification cartridge in one of the plurality of cartridge receivers.
21. The processing system of 18 further comprising at least one spring loaded grip configured to secure the purification cartridge in one of the plurality of cartridge receivers.
22. The processing system of 18 wherein the controller is further configured to control the system so as to process two different samples at one time.