Preparation of regenerative tissue scaffolds
View Patent ↗Devices and methods for treating or repairing a tissue or organ defect or injury are provided. The devices can include tissue scaffolds produced from acellular tissue matrices and polymers, which have a stable three-dimensional shape and elicit a limited immunologic or inflammatory response.
1. A tissue scaffold, comprising:
an acellular tissue matrix (ATM) comprising a group of ATM particulates; and
a polymer;
wherein the ATM particulates are encased in the polymer to form a stable three-dimensional tissue scaffold for tissue regeneration, and the tissue scaffold has a reduced immunological or inflammatory response when implanted in a human than the polymer alone; and
the polymer has been dissolved in a solvent to form a polymer solution, the ATM particulates have been submerged in and mixed with the polymer solution to form a moldable mixture, and the moldable mixture has been dried to produce a polymer scaffold completely surrounding the ATM particulates.
2. The tissue scaffold of claim 1 , wherein the polymer comprises a synthetic polymer.
3. The tissue scaffold of claim 1 , wherein the polymer comprises a polycaprolactone.
4. The tissue scaffold of claim 1 , wherein the polymer is a poly4-hydroxybutyrate.
5. The tissue scaffold of claim 1 , wherein the polymer comprises a benzyl ester derivative of hyaluronic acid.
6. The tissue scaffold of claim 1 , wherein the polymer comprises chitosan.
7. The tissue scaffold of claim 1 , wherein the ATM comprises a dermal ATM.
8. The tissue scaffold of claim 7 , wherein the dermal ATM is a human tissue matrix.
9. The tissue scaffold of claim 7 , wherein the dermal ATM is a porcine tissue matrix.
10. The tissue scaffold of claim 1 , wherein the ATM is a cartilage tissue matrix.
11. The tissue scaffold of claim 10 , wherein the cartilage tissue matrix comprises a human cartilage.
12. The tissue scaffold of claim 10 , wherein the cartilage tissue matrix comprises a porcine cartilage.
13. The tissue scaffold of claim 1 , wherein the ATM is a bone tissue matrix.
14. The tissue scaffold of claim 13 , wherein the bone tissue matrix is derived from human bone.
15. The tissue scaffold of claim 13 , wherein the bone tissue matrix is derived from porcine bone.
16. The tissue scaffold of claim 1 , wherein the ATM includes tissue matrices derived from two or more different types of tissues.
17. The tissue scaffold of claim 16 , wherein the two or more different types of tissues include cartilage and demineralized bone.
18. The tissue scaffold of claim 1 , wherein the solvent includes at least one of dioxane, N-methyl-2-pyrrolidone, DMSO, and acetic acid.
19. The tissue scaffold of claim 1 , wherein drying includes a drying process selected from at least one of freeze drying, air drying, heated drying, drying in an argon or nitrogen atmosphere, and vacuum assisted drying.
20. A tissue scaffold, comprising:
an acellular tissue matrix (ATM) comprising a group of ATM particulates; and
a polymer;
wherein the ATM particulates are encased in the polymer to form a stable three-dimensional tissue scaffold for tissue regeneration, and the tissue scaffold has a reduced immunological or inflammatory response when implanted in a human than the polymer alone; and
wherein the ATM particulates have been mixed with a polymer solution to form a moldable mixture, and the moldable mixture has been dried to produce the stable three-dimensional tissue scaffold.
21. The tissue scaffold of claim 20 , wherein the ATM comprises a dermal ATM.
22. The tissue scaffold of claim 21 , wherein the dermal ATM is a human tissue matrix.
23. The tissue scaffold of claim 21 , wherein the dermal ATM is a porcine tissue matrix.