Host, transformant and method for producing the transformant and method for producing O-glycosylated heterologous protein
View Patent ↗The object is to provide a transformant which can produce a heterologous protein having a structurally controlled O-linked sugar chain having an O-Man-Gal disaccharide structure, a method for producing the transformant by using Schizosaccharomyces pombe as the host, and provide a host for producing the transformant and a method for producing an O-glycosylated heterologous protein. An Schizosaccharomyces pombe host having no omh1 gene or an inactivated omh1 gene in its chromosomes for producing an O-glycosylated heterologous protein having an O-linked sugar chain having an O-Man-Gal disaccharide structure by expression of the heterologous protein by a genetic engineering technique and subsequent glycosylation of the expressed heterologous protein. A transformant from the host, a method for producing the transformant and a method for producing an O-glycosylated heterologous protein by using the transformant.
1. A Schizosaccharomyces pombe host having no omh1 gene or an inactivated omh1 gene and comprising a heterologous gene, wherein the heterologous gene expresses an O-glycosylated heterologous protein having an O-linked sugar chain and an O-Man-Gal disaccharide structure.
2. The Schizosaccharomyces pombe host according to claim 1 , wherein the heterologous gene is a recombinant fusion further comprising a gene encoding a secretion signal functional in the Schizosaccharomyces pombe linked to the 5′ end of the heterologous gene.
3. The Schizosaccharomyces pombe host according to claim 1 , wherein the heterologous protein, in wild type form, produced by the transformant is an O-glycosylated protein.
4. A method for producing the Schizosaccharomyces pombe host according to claim 1 , the method comprising introducing the heterologous gene into the Schizosaccharomyces pombe host having no omh1 gene or an inactivated omh1 gene.
5. The method according to claim 4 , wherein the heterologous gene is a recombinant fusion further comprising a gene encoding a secretion signal functional in Schizosaccharomyces pombe linked to-the 5′ end of the gene.
6. The method according to claim 4 , wherein the heterologous protein, in wild type form, is an O-glycosylated protein.
7. A method for producing an O-glycosylated heterologous protein, the method comprising culturing the Schizosaccharomyces pombe host of claim 1 to produce an O-glycosylated heterologous protein having an O-linked sugar chain and an O-Man-Gal disaccharide structure.
8. The method for producing an O-glycosylated heterologous protein according to claim 7 , wherein the O-linked sugar chain of the produced O-glycosylated heterologous protein has an O-Man-Gal disaccharide structure irrespective of whether a wild type of the heterologous protein is glycosylated and the structure of the sugar chain in the wide type of the heterologous protein.
9. The method for producing an O-glycosylated heterologous protein according to claim 7 , further comprising recovering the O-glycosylated heterologous protein.
10. The Schizosaccharomyces pombe host according to claim 1 , which has no omh1 gene.
11. The Schizosaccharomyces pombe host according to claim 1 , which has an inactivated omh1 gene.