RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
View Patent ↗The present invention concerns methods and reagents useful in modulating gene expression in a variety of applications, including use in therapeutic, diagnostic, target validation, and genomic discovery applications. Specifically, the invention relates to synthetic chemically modified small nucleic acid molecules, such as short interfering nucleic acid (siNA), short interfering RNA (siRNA), double-stranded RNA (dsRNA), micro-RNA (miRNA), and short hairpin RNA (shRNA) molecules capable of mediating RNA interference (RNAi) against target nucleic acid sequences. The small nucleic acid molecules are useful in the treatment of any disease or condition that responds to modulation of gene expression or activity in a cell, tissue, or organism.
1. A chemically modified double-stranded short interfering nucleic acid molecule comprising a sense strand and an antisense strand that mediates RNA interference (RNAi) against a target RNA sequence, wherein:
a) each strand of the nucleic acid molecule is independently 18 to 24 nucleotides in length;
b) the antisense strand is complementary to the target RNA sequence;
c) the sense strand comprises 10 or more 2′-deoxy, 2′-O-methyl, 2′-deoxy-2′-fluoro, or universal base modified nucleotides, and all of the pyrimidine nucleotides present in the sense strand are 2′-deoxy, 2′-O-methyl, or 2′-deoxy-2′-fluoro pyrimidine nucleotides; and
d) the antisense strand comprises 10 or more 2′-deoxy, 2′-O-methyl, 2′-deoxy-2′-fluoro, or universal base modified nucleotides, and all of the pyrimidine nucleotides present in the antisense strand are 2′-deoxy, 2′-O-methyl, or 2′-deoxy-2′-fluoro pyrimidine nucleotides.
2. The nucleic acid molecule of claim 1 , wherein the sense strand comprises a terminal cap modification at the 3′-end, the 5′-end, or both 3′ and 5′-ends of the sense strand.
3. The nucleic acid molecule of claim 1 , wherein the sense strand comprises one or more phosphorothioate internucleotide linkages.
4. The nucleic acid molecule of claim 1 , wherein the antisense strand comprises one or more phosphorothioate internucleotide linkages.
5. A composition comprising the nucleic acid molecule of claim 1 and a pharmaceutically acceptable carrier or diluent.