Reducing levels of nicotinic alkaloids in plants
View Patent ↗Two genes, A622 and NBB1, can be influenced to achieve a decrease of nicotinic alkaloid levels in plants. In particular, suppression of one or both of A622 and NBB1 may be used to decrease nicotine in tobacco plants.
1. A reduced nicotinic alkaloid tobacco plant comprising a polynucleotide sequence in sense orientation or antisense orientation that suppresses expression of NBB1 in the plant or an siRNA molecule comprising a polynucleotide sequence that suppresses expression of NBB1 in the plant, wherein the polynucleotide sequence comprises SEQ ID NO: 3 or a fragment thereof, and wherein NBB1 is suppressed in the plant.
2. A product produced from the tobacco plant of claim 1 , wherein the product comprises said polynucleotide.
3. The product of claim 2 , wherein the product is selected from the group consisting of smoking cessation products, cigarettes, cigarette tobacco, cigars, cigar tobacco, snuffs, pipe tobacco and chewing tobacco.
4. The product of claim 2 , wherein the product is selected from the group consisting of a food product, food ingredient, feed product, feed ingredient, nutritional supplement, and biofuels.
5. An isolated plant cell from the tobacco plant of claim 1 , wherein said cell comprises said polynucleotide.
6. The tobacco plant of claim 1 , wherein the polynucleotide sequence comprises SEQ ID NO: 3.
7. The tobacco plant of claim 1 , wherein the nicotinic alkaloid is nicotine.
8. The tobacco plant of claim 1 , wherein the nicotinic alkaloid is anatabine.