Optical apparatus
View Patent ↗An optical apparatus for illuminating particles and for detecting emissions from illuminated particles in a flow cytometer having an optical configuration aligned with a flow or particles.
1. An optical apparatus comprising:
a flow source to produce a flow of sperm cells to be analyzed along a central axis, the flow source adapted to direct a flow of sperm cells through an inspection zone;
a source of electromagnetic radiation for irradiating sperm cells at the inspection zone;
an optical configuration having an optical axis, wherein the optical configuration focuses said electromagnetic radiation on the inspection zone and collects at least a portion of any electromagnetic radiation produced or deflected as a result of the illumination of the flow of sperm cells, wherein the optical axis of the optical configuration is coaxial with the central axis of the flow of sperm cells;
a sensor for sensing said electromagnetic radiation collected by the optical configuration;
a processor to derive predetermined information from the collected electromagnetic radiation; and
a correlator to correlate said information to sperm cells downstream of the inspection zone.
2. The optical apparatus of claim 1 wherein the optical configuration comprises an objective lens for focusing at least a portion of any electromagnetic radiation produced or deflected as a result of the illumination of the flow of sperm cells onto the sensor.
3. The optical apparatus of claim 1 wherein the optical configuration comprises a reflective surface with an optical axis which is coaxial with the central axis of the flow of sperm cells.
4. The optical apparatus of claim 1 wherein optical configuration comprises a reflector with an optical axis which is coaxial with the central axis of the flow of sperm cells and an objective lens.
5. The optical apparatus of claim 4 wherein the optical axis of the objective lens is coaxial with the central axis of the flow of sperm cells and an objective lens.
6. The optical apparatus of claim 4 further comprising a second reflector for reflecting light from the first reflector through the objective lens to the sensor.
7. The optical apparatus of claim 4 wherein the reflector comprises a reflector with one or more foci on an optical axis, wherein the optical axis is coaxial with the central axis of the flow of sperm cells.
8. The optical apparatus of claim 1 wherein optical configuration comprises a reflective surface with an optical axis or an objective lens with an optical axis.
9. The optical apparatus of claim 1 where the light collected by the optical configuration is unaffected by sperm orientation.
10. The optical apparatus of claim 9 wherein the optical apparatus comprises a sperm sorter without a sperm orienting feature.
11. The optical apparatus of claim 1 wherein the sperm sorter includes an orienting feature.
12. The optical apparatus of claim 1 wherein the optical apparatus is a flow cytometer.
13. An optical apparatus comprising:
a flow path with a central axis for flowing sperm cells to be analyzed through an inspection zone;
a source of electromagnetic radiation for irradiating sperm cells at the inspection zone;
an optical configuration, having an optical axis, wherein the optical configuration focuses said electromagnetic radiation on the inspection zone and collects at least a portion of any electromagnetic radiation produced or deflected as a result of the illumination of the flow of sperm cells, wherein the optical axis of the optical configuration is coaxial with the central axis of the flow of sperm cells;
a sensor for sensing electromagnetic radiation collected by the optical configuration;
a processor to derive predetermined information from the collected radiation; and
a correlator to correlate said information to sperm cells downstream of the inspection zone.
14. A method of analyzing comprising the steps of:
a. flowing sperm cells to be analyzed along a central axis through an inspection zone;
b. focusing a source of electromagnetic radiation at the inspection zone with an optical configuration having an optical axis;
c. illuminating the sperm cells at the inspection zone with the focused electromagnetic radiation;
d. collecting at least a portion of any electromagnetic radiation produced or deflected from the illumination of the flow of sperm cells with said optical configuration having an optical axis;
e. sensing a portion of the electromagnetic radiation produced from the illumination of the flow of sperm cells wherein the optical axis of the optical configuration is substantially aligned with the central axis along with the sperm cells flow; and
f. sorting sperm cells based on the sensed illumination.
15. The method according to claim 14 wherein the substantially aligned optical axis is coaxial with the central axis along which the sperm cells flow.