Regulating nutrient allocation in plants
View Patent ↗The invention provides coding and promoter sequences for a VS-1 and AP-2 gene, which affects the developmental process of senescence in plants. Vectors, transgenic plants, seeds, and host cells comprising heterologous VS-1 and AP-2 genes are also provided. Additionally provided are methods of altering nutrient allocation and composition in a plant using the VS-1 and AP-2 genes.
1. An expression cassette comprising a polynucleotide molecule comprising a sequence selected from the group consisting of:
(a) a sequence encoding SEQ ID NO: 2 or a polypeptide at least 98% identical to SEQ ID NO: 4, wherein the polypeptide regulates plant leaf senescence;
(b) a sequence comprising SEQ ID NO: 1 or 3; and
(c) a sequence complementary to the full length of (a) or (b),
the expression cassette further comprising a heterologous promoter functional in plants operably linked to the polynucleotide molecule.
2. The expression cassette of claim 1 , wherein the polynucleotide molecule comprises the sequence of SEQ ID NO:1 or SEQ ID NO:3.
3. A recombinant vector comprising the expression cassette of claim 1 .
4. The recombinant vector of claim 3 , further comprising at least one additional sequence chosen from the group consisting of: a regulatory sequence, a selectable marker, a leader sequence and a terminator.
5. The recombinant vector of claim 4 , wherein the additional sequence is heterologous to the polynucleotide molecule.
6. The recombinant vector of claim 3 , wherein the promoter is a tissue-specific promoter.
7. The recombinant vector of claim 3 , wherein the promoter directs expression in leaf tissue.
8. A transgenic plant comprising the expression cassette of claim 1 .
9. The transgenic plant of claim 8 , further defined as a dicotyledonous plant.
10. The transgenic plant of claim 8 , further defined as a poplar, a willow, a eucalyptus, a hemp, a Medicago sp., a Lotus sp., a Trifolium sp., a Melilotus sp., a Vinca sp., a Glycine sp., a Nicotiana sp., a Vitis sp., an Arabidopsis sp. or a Ricinus sp.
11. The transgenic plant of claim 8 , further defined as a monocotyledonous plant.
12. The transgenic plant of claim 8 , further defined as a rice, a wheat, a barley, a maize, a switchgrass, an oat, a sugarcane, a rye or a sorghum.
13. The transgenic plant of claim 8 , further defined as an R 0 transgenic plant.
14. The transgenic plant of claim 8 , further defined as a progeny plant of any generation of an R 0 transgenic plant, wherein the transgenic plant has the nucleic acid molecule from the R 0 transgenic plant.
15. A seed of the transgenic plant of claim 8 , wherein the seed comprises the expression cassette.
16. The seed of claim 15 , wherein nitrogen content is increased relative to that found in seed of an otherwise isogenic plant lacking the recombinant.
17. A host cell transformed with the recombinant vector of claim 3 .
18. The host cell of claim 17 , wherein said host cell is a plant cell.
19. A method of altering the distribution of one or more nutrients in a plant, the method comprising growing the plant, wherein the plant comprises the expression cassette of claim 1 , wherein the selected sequence is expressed and alters the distribution of one or more nutrients in the plant when compared to an otherwise isogenic plant that lacks the polynucleotide molecule.
20. The method of claim 19 , wherein the one or more nutrients comprises protein.
21. The method of claim 19 , wherein the plant is an R 0 transgenic plant.
22. The method of claim 19 , wherein the plant is a progeny plant of any generation of an R 0 transgenic plant, wherein the transgenic plant comprises the polynucleotide molecule.
23. The method of claim 19 , wherein the altered distribution of one or more nutrients is a decrease of one or more nutrients in the leaves.
24. The method of claim 19 , wherein the plant has altered development or morphology when compared to an otherwise isogenic plant that lacks the polynucleotide molecule.
25. The method of claim 24 , wherein the altered development is altered leaf senescence.
26. A method of producing plant biomass, the method comprising:
(a) obtaining the plant of claim 8 ;
(b) growing said plant under plant growth conditions to produce plant tissue from the plant; and
(c) preparing biomass from said plant tissue.
27. The method of claim 26 , wherein preparing biomass comprises harvesting said plant tissue.
28. The method of claim 26 , further comprising using the biomass for biofuel.