Compositions for isolating a target analyte from a heterogeneous sample
View Patent ↗The invention generally relates to compositions that include sets of magnetic particles, members of each set being conjugated to an antibody specific for a pathogen.
1. A composition for isolating pathogen from a heterogeneous sample, the composition comprising:
a plurality of sets of magnetic particles, members of different sets being conjugated to different antibodies that are specific for a pathogen, wherein at least two of the sets are at different concentrations; and
a buffer that substantially prevents lysis of blood cells and reduces particle aggregation.
2. The composition of claim 1 , wherein each set is present at a concentration designed for detection of a specific pathogen in said sample.
3. The composition of claim 1 , wherein the particles are differently labeled.
4. The composition of claim 3 , wherein the label is an optical label.
5. The composition of claim 4 , wherein the optical label is a fluorescent label.
6. The composition of claim 1 , wherein the antibodies are monoclonal antibodies.
7. The composition of claim 1 , wherein the antibodies are polyclonal antibodies.
8. The composition of claim 1 , wherein the pathogen are gram positive bacteria.
9. The composition of claim 1 , wherein the pathogen are gram negative bacteria.
10. The composition of claim 1 , wherein the pathogen are gram positive and gram negative bacteria.
11. A plurality of magnetic particles within a buffer that substantially prevents lysis of blood cells and reduces particle aggregation, wherein the plurality is differently functionalized so that the plurality of particles bind to a plurality of different pathogens in a heterogeneous sample, wherein at least some of the differently functionalized particles are at different concentrations.
12. The particles of claim 11 , wherein the particles are differently labeled.
13. The particles of claim 12 , wherein the label is an optical label.
14. The particles of claim 13 , wherein the optical label is a fluorescent label.
15. The particles of claim 11 , wherein the antibodies are monoclonal antibodies.
16. The particles of claim 11 , wherein the antibodies are polyclonal antibodies.
17. The particles of claim 11 , wherein the pathogen are gram positive bacteria.
18. The particles of claim 11 , wherein the pathogen are gram negative bacteria.
19. The particles of claim 11 , wherein the pathogen are gram positive and gram negative bacteria.
20. A composition for isolating bacteria from a heterogeneous sample, the composition comprising:
a plurality of sets of magnetic particles, members of different sets being conjugated to different antibodies that are specific for a bacteria, wherein at least two of the sets are at different concentrations; and
a buffer that substantially prevents lysis of blood cells and reduces particle aggregation.