Assays that detect T1R expressing cells
View Patent ↗Newly identified mammalian taste-cell-specific G protein-coupled receptors, and the genes and cDNA encoding said receptors are described. Specifically, T1R G protein-coupled receptors active in taste signaling, and the genes and cDNA encoding the same, are described, along with methods for isolating such genes and for isolating and expressing such receptors. Methods for representing taste perception of a particular tastant in a mammal are also described, as are methods for generating novel molecules or combinations of molecules that elicit a predetermined taste perception in a mammal, and methods for simulating one or more tastes. Further, methods for stimulating or blocking taste perception in a mammal are also disclosed.
1. A method of identifying T1R expressing cells comprising detecting cells that express a nucleic acid that encodes a polypeptide that is at least 90% identical to the polypeptide in SEQ ID NO:4, the method comprising:
(i) contacting one or more cells or nucleic acids isolated therefrom with a nucleic acid probe that specifically binds to a cDNA or cRNA that encodes a polypeptide that is at least 90% identical to the polypeptide in SEQ ID NO;4;
(ii) detecting whether said nucleic acid probe specifically binds to a cDNA or cRNA that encodes for said polypeptide that is at least 90% identical to the polypeptide in SEQ ID NO:4; and
(iii) identifying the one or more cells as T1R expressing cells if they comprise nucleic acid detected in the binding reaction of (ii).
2. The method of claim 1 which uses a probe that detects the expression of a nucleic acid having the sequence of SEQ ID NO;20.
3. The method of claim 1 that uses a probe that is at least 20 nucleotide bases in length.
4. The method of claim 1 that detects the expression of the nucleotide sequence of SEQ ID NO: 3.
5. The method of claim 1 wherein said detected T1R expressing cell is isolated.
6. The method of claim 1 , wherein the one or more cells are mammalian cells.
7. The method of claim 1 , wherein the one or more cells are human cells.
8. The method of claim 1 , wherein the one or more cells are recombinant cells.