IP Library Granted Patent US 8,802,369
Granted Patent B2
US 8,802,369 · App. 13/098,613 · Granted Aug 12, 2014

Gel having biosubstance fixed thereto and microarray utilizing the gel

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Quick Facts
Patent No.
US 8,802,369
App. No.
13/098,613
Granted
Aug 12, 2014
Kind
B2
Abstract

The present invention provides a biological substance-immobilized gel which comprises a gel containing 2%-7% by mass of N,N-dimethylacrylamide and a biological substance immobilized on and/or in the gel.

Claims (13)

1. A method for detecting a target to be measured, which comprises

reacting an analyte with a biological substance-immobilized gel microarray which comprises a biological substance-immobilized gel which comprises a gel containing 2%-7% by mass of N,N-dimethylacrylamide and a biological substance immobilized on and/or in the gel, wherein the gel is arranged in multiple compartments; and

detecting the target in the analyte based on uniform fluorescence in the gel,

wherein the gel comprises a cross-linking agent in an amount of 0.1% to 1.5% by mass.

2. The method according to claim 1 , wherein the target to be measured is a nucleic acid.

3. The method according to claim 2 , wherein the nucleic acid is 100 nucleotides or less in length.

4. The method according to claim 1 , wherein each compartment has a surface area of 10 −6 m 2 or less.

5. The method according to claim 1 , wherein the compartments are formed by slots or through holes.

6. The method according to claim 1 , wherein the biological substance-immobilized gel microarray is obtained by tying a plurality of gel-filled hollow tubes in a bundle and cutting the tube bundle in a direction intersecting with the longitudinal direction of the tubes.

7. The method according to claim 6 , wherein the hollow tubes are hollow fibers.

8. The method according to claim 1 , wherein the cross-linking agent is a multifunctional monomer having at least two ethylenically unsaturated bonds.

9. The method according to claim 8 , wherein the cross-linking agent is methylenebisacrylamide.

10. The method according to claim 1 , wherein the gel comprises 2.7 to 4.5% by mass of N,N-dimethylacrylamide.

Assignments (1)
CHANGE OF NAME Recorded Sep 5, 2017
From: MITSUBISHI RAYON CO., LTD.
To: MITSUBISHI CHEMICAL CORPORATION
Reel/Frame 043750/0834 →