Enhanced production of fatty acid derivatives
View Patent ↗Genetically engineered cells and microorganisms are provided that produce fatty alcohols from the fatty acid biosynthetic pathway, as well as methods of their use.
1. A method of producing a fatty alcohol composition, which method comprises the steps of:
(a) obtaining a genetically engineered microbial cell comprising polynucleotide sequences encoding polypeptides having (i) acetyl-CoA carboxylase (accABCD, EC 6.4.1.2) activity and (ii) fatty alcohol forming activity selected from the group consisting of alcohol forming acyl-CoA reductase (FAR, EC 1.1.1.*), acyl-CoA reductase (EC 1.2.1.50) and alcohol dehydrogenase (EC 1.1.1.1),
(b) culturing the genetically engineered cell in a culture medium containing a carbon source under conditions effective to overexpress the polynucleotide sequences encoding the polypeptides having accABCD activity and fatty alcohol forming activity,
(c) producing a fatty alcohol in the genetically engineered microbial cell, and
(d) optionally recovering the fatty alcohol composition from the cell culture medium.
2. The method of producing a fatty alcohol composition according to claim 1 , wherein the cell is further engineered to comprise a polynucleotide sequence encoding a polypeptide having (iii) thioesterase activity (EC 3.1.2.- or EC 3.1.1.5) and cultured in a culture medium containing a carbon source under conditions effective to overexpress the polynucleotide sequences encoding the polypeptides having acetyl-CoA carboxylase activity, fatty alcohol forming activity, and thioesterase activity.
3. The method of producing a fatty alcohol composition according to claim 2 , wherein the fatty alcohol forming activity is alcohol forming acyl-CoA reductase (FAR, EC 1.1.1.*).
4. The method of producing a fatty alcohol composition according to claim 2 , wherein the fatty alcohol forming activity is acyl-CoA reductase (EC 1.2.1.50).
5. The method of producing a fatty alcohol composition according to claim 1 , wherein the fatty alcohol composition comprises one or more saturated fatty alcohols.
6. The method of producing a fatty alcohol composition according to claim 1 , wherein the fatty alcohol composition comprises one or more unsaturated fatty alcohols.
7. The method of producing a fatty alcohol composition according to claim 1 , wherein the fatty alcohol composition comprises one or more of C 10 , C 12 , C 14 , C 16 , and C 18 fatty alcohols.
8. The method of producing a fatty alcohol composition according to claim 7 , wherein the fatty alcohol composition comprises C 12 and C 14 fatty alcohols.
9. The method of producing a fatty alcohol composition according to claim 2 , wherein the fatty alcohol composition comprises unsaturated fatty alcohols.
10. The method of producing a fatty alcohol composition according to claim 2 , wherein the fatty alcohol composition comprises saturated fatty alcohols.
11. The method of producing a fatty alcohol composition according to claim 2 , wherein the fatty alcohol composition comprises straight chain or branched chain fatty alcohols.
12. The method of producing a fatty alcohol composition according to claim 2 , wherein the fatty alcohol composition comprises a fatty alcohol having a double bond at position 7 in the carbon chain (between C 7 and C 8 ) from the reduced end of the fatty alcohol.
13. A surfactant comprising a fatty alcohol composition produced by the method of claim 2 .
14. A detergent comprising a fatty alcohol composition produced by the method of claim 2 .
15. A surfactant comprising a fatty alcohol composition produced according to the method of claim 1 .
16. A detergent comprising a fatty alcohol composition produced according to the method of claim 1 .