Microorganisms and methods for the biosynthesis of butadiene

1. A non-naturally occurring microbial organism, said microbial organism having a butadiene pathway and comprising at least one exogenous nucleic acid encoding a butadiene pathway enzyme expressed in a sufficient amount to produce butadiene, said butadiene pathway comprising an acetyl-CoA:acetyl-CoA acyltransferase, an acetoacetyl-CoA reductase, a 3-hydroxybutyryl-CoA dehydratase, a crotonyl-CoA reductase (aldehyde forming), a crotonaldehyde reductase (alcohol forming), a crotyl alcohol kinase, a 2-butenyl-4-phosphate kinase and a butadiene synthase.

2. The non-naturally occurring microbial organism of claim 1 , wherein said microbial organism comprises one, two, three, four, five, six, seven, or eight exogenous nucleic acids each encoding a butadiene pathway enzyme.

3. The non-naturally occurring microbial organism of claim 1 , wherein said at least one exogenous nucleic acid is a heterologous nucleic acid.

4. The non-naturally occurring microbial organism of claim 1 , wherein said non-naturally occurring microbial organism is in a substantially anaerobic culture medium.

5. A method for producing butadiene, comprising culturing the non-naturally occurring microbial organism of claim 1 under conditions and for a sufficient period of time to produce butadiene.

6. The method of claim 5 , wherein said non-naturally occurring microbial organism is in a substantially anaerobic culture medium.

7. The non-naturally occurring microbial organism of claim 1 , wherein said microbial organism comprises one exogenous nucleic acids encoding a butadiene pathway enzyme.

8. The non-naturally occurring microbial organism of claim 1 , wherein said microbial organism comprises two exogenous nucleic acids each encoding a butadiene pathway enzyme.

9. The non-naturally occurring microbial organism of claim 1 , wherein said microbial organism comprises three exogenous nucleic acids each encoding a butadiene pathway enzyme.

10. The non-naturally occurring microbial organism of claim 1 , wherein said microbial organism comprises four exogenous nucleic acids each encoding a butadiene pathway enzyme.

11. The non-naturally occurring microbial organism of claim 1 , wherein said microbial organism comprises five exogenous nucleic acids each encoding a butadiene pathway enzyme.

12. The non-naturally occurring microbial organism of claim 1 , wherein said microbial organism comprises six exogenous nucleic acids each encoding a butadiene pathway enzyme.

13. The non-naturally occurring microbial organism of claim 1 , wherein said microbial organism comprises seven exogenous nucleic acids each encoding a butadiene pathway enzyme.

14. The non-naturally occurring microbial organism of claim 1 , wherein said microbial organism comprises eight exogenous nucleic acids each encoding a butadiene pathway enzyme.