Enhanced Clarification Media
Media and devices, such as depth filters including such media, wherein the media is impregnated with a polymer such as a polyallylamine. The resulting device offers strong binding of protein impurities and superior removal of host cell proteins from biological samples.
1 . A porous sorptive media comprising cellulose impregnated with a crosslinked polymer having attached primary amine groups.
2 . The porous sorptive media of claim 1 , further comprising diatomaceous earth.
3 . The porous coated media of claim 1 , wherein said crosslinked polymer comprises polyallylamine or a protonated polyallylamine.
4 . The porous sorptive media of claim 1 , wherein said crosslinked polymer comprises a copolymer or block copolymer containing polyallylamine or a protonated polyallylamine.
5 . The porous sorptive media of claim 1 , wherein said cellulose comprises cellulose fibers.
6 . The porous sorptive media of claim 2 , wherein said cellulose and said diatomaceous earth are held together by a binder.
7 . A depth filter comprising a housing containing a matrix of cellulose fibers impregnated with a crosslinked polymer having attached primary amine groups.
8 . The depth filter of claim 7 , wherein said matrix further comprises diatomaceous earth.
9 . The depth filter of claim 7 , wherein said crosslinked polymer comprises polyallylamine or a protonated polyallylamine.
10 . The depth filter of claim 7 , wherein said crosslinked polymer comprises a copolymer or block copolymer containing polyallylamine or a protonated polyallylamine.
11 . The depth filter of claim 8 , wherein said cellulose fibers and said diatomaceous earth are held together by a binder.
12 . A method of removing impurities from a biological sample, comprising filtering said sample through porous sorptive media comprising cellulose impregnated with a crosslinked polymer having attached primary amine groups.
13 . The method of claim 12 , wherein said biological sample comprises a solution having a pH of about 7.5.
14 . The method of claim 12 , wherein said biological sample comprises a solution having a conductivity of about 10.4 mS/cm.