Nanocomposite hyaluronic acid-clay based hydrogels
View Patent ↗Hydrogels formed from hyaluronic acid, a nanoclay and gelatin-type A. The hyaluronic acid is preferably dissolved in a cell culture medium containing amino acids, salts, glucose, vitamins and an antibiotic to form a hyaluronic acid solution. The gelatin-type A has a weight/volume of from about 0.02% to 0.08%. The nanoclay is exfoliated in deionized water and preferably includes SiO 2 , MgO, Na 2 O and Li 2 O.
1. A hydrogel comprising:
hyaluronic acid (“HA”);
a nanoclay; and
gelatin-type A
wherein the HA is dissolved in a cell culture medium containing amino acids, salts, glucose, vitamins and an antibiotic to form a HA solution;
wherein the hydrogel comprises 5-30 percent by weight HA, 5-30 percent by weight gelatin-type A, and about 40-90 percent by weight nanoclay; and
wherein the nonclay comprised about 55-65% SiO 2 , 20-35% MgO, 1-5%, Na 2 O and 0.2-2% LiO 2 O.
2. The hydrogel according to claim 1 , wherein the nanoclay is exfoliated in deionized water.
3. The hydrogel according to claim 1 , wherein the gelatin-type A has a weight/volume of from about 0.02% to 0.08%.
4. The hydrogel according to claim 3 , wherein the HA solution and gelatin-type A have a volume ratio of from about 8:5 to about 5:8.
5. The hydrogel according to claim 3 , wherein the HA solution and gelatin-type A have a volume ratio of about 1:1.
6. The hydrogel according to claim 3 , wherein the nanoclay has a weight/volume of from about 0.01% to about 0.08%.
7. The hydrogel according to claim 3 , wherein the volume ratio of HA/gelatin-type A mixture to clay is from about 1:5 to about 1:20.
8. The hydrogel according to claim 3 , wherein the hydrogel comprises 10-25 percent by weight HA, 10-25 percent by weight gelatin and about 50-80 percent by weight nanoclay.