IP Library Granted Patent US 9,068,215
Granted Patent B2
US 9,068,215 · App. 13/107,503 · Granted Jun 30, 2015

Interconnected bioreactors

Inventor: Sarfaraz K. Niazi (Deerfield, IL)
Assignee: Therapeutic Proteins International, LLC
C12Q3/00C12M23/14C12M23/58C12M27/16
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Quick Facts
Patent No.
US 9,068,215
App. No.
13/107,503
Granted
Jun 30, 2015
Kind
B2
Abstract

A method of homogenously mixing the contents of a plurality of bioreactors by providing a receiving container capable of holding an appropriate quantity of the liquid and repeatedly raising and lowering the receiving container to a position above or below the position of the bioreactors resulting in mixing the contents of the bioreactors.

Claims (13)

1. A method of homogenously mixing the contents of a plurality of bioreactors comprising:

(a) providing a plurality of bioreactors, 1 through nth, in a series, installed on a same plane either in a series or in a circular pattern, the bioreactors comprising a bioreactor container with at least two surfaces and two opposing sides, to hold a liquid further comprising a nutrient media and a biological culture, a means of gassing the liquid further comprising a gas inlet, a gas sterilizing filter and a gas sparging filter, a gas exhaust, at least one nutrient media inlet/outlet, at least one liquid ports installed on the bottom with means of closing or opening the liquid port, a means of agitating the nutrient media, a plurality of sensors installed in the bioreactor container with at least a sensor for measuring the weight of the bioreactor container;

(b) providing a receiving container capable of holding an appropriate quantity of the liquid wherein the receiving container further comprises a support capable of being raised or lowered;

(c) connecting the liquid port of each bioreactor to the receiving container;

(d) operating the bioreactors;

(e) opening all liquid ports;

(f) lowering the receiving container support to a position below the position of bioreactors;

(g) measuring the increase in the weight of the receiving bioreactor to a pre-determined level;

(h) raising the receiving container support to a position higher than the bioreactors;

(i) measuring the decrease in the weight of the receiving container;

(j) repeating the steps (f) to (i) periodically or continuously.

2. The method of homogenously mixing according to claim 1 , wherein the bioreactors are arranged on a variable plane.

3. The method of homogenously mixing according to claim 1 , wherein the receiving container is rocked, shaken or stirred continuously or intermittently.

Assignments (2)
CHANGE OF NAME Recorded Feb 2, 2017
From: THERAPEUTIC PROTEINS INTERNATIONAL, LLC
To: ADELLO BIOLOGICS, LLC
Reel/Frame 041605/0782 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 27, 2012
From: NIAZI, SARFARAZ K.
To: THERAPEUTIC PROTEINS INTERNATIONAL, LLC
Reel/Frame 028853/0162 →
Continuity (1)
Related Publication 20110217690A1 · Sep 8, 2011