Chimeric OspA genes, proteins, and methods of use thereof
The invention relates to the development of chimeric OpsA molecules for use in a new Lyme vaccine. More specifically, the chimeric OspA molecules comprise the proximal portion from one OspA serotype, together with distal portion from another OspA serotype, while retaining antigenic properties of both of the parent polypeptides. The chimeric OspA molecules are delivered alone or in combination to provide protection against a variety of Borrelia genospecies. The invention also provides methods for administering the chimeric OspA molecules to a subject in the prevention and treatment of Lyme disease or borreliosis.
1. An isolated polypeptide comprising an amino acid sequence set forth in SEQ ID NO: 6.
2. A composition comprising the isolated polypeptide of claim 1 and a pharmaceutically acceptable carrier.
3. An immunogenic composition comprising the composition of claim 2 and an adjuvant.
4. A vaccine composition comprising the composition claim 2 and an adjuvant.
5. An isolated polypeptide comprising an amino acid sequence having at least 90 percent sequence identity to the amino acid sequence set forth in SEQ ID NO: 6.
6. The isolated polypeptide of claim 5 comprising an amino acid sequence having at least 91 percent sequence identity to the amino acid sequence set forth in SEQ ID NO: 6.
7. The isolated polypeptide of claim 5 comprising an amino acid sequence having at least 92 percent sequence identity to the amino acid sequence set forth in SEQ ID NO: 6.
8. The isolated polypeptide of claim 5 comprising an amino acid sequence having at least 93 percent sequence identity to the amino acid sequence set forth in SEQ ID NO: 6.
9. The isolated polypeptide of claim 5 comprising an amino acid sequence having at least 94 percent sequence identity to the amino acid sequence set forth in SEQ ID NO: 6.
10. The isolated polypeptide of claim 5 comprising an amino acid sequence having at least 95 percent sequence identity to the amino acid sequence set forth in SEQ ID NO: 6.
11. The isolated polypeptide of claim 5 comprising an amino acid sequence having at least 96 percent sequence identity to the amino acid sequence set forth in SEQ ID NO: 6.
12. The isolated polypeptide of claim 5 comprising an amino acid sequence having at least 97 percent sequence identity to the amino acid sequence set forth in SEQ ID NO: 6.
13. The isolated polypeptide of claim 5 comprising an amino acid sequence having at least 98 percent sequence identity to the amino acid sequence set forth in SEQ ID NO: 6.
14. The isolated polypeptide of claim 5 comprising an amino acid sequence having at least 99 percent sequence identity to the amino acid sequence set forth in SEQ ID NO: 6.
15. The isolated polypeptide of claim 1 consisting of the amino acid sequence set forth in SEQ ID NO: 6.
16. A composition comprising the isolated_polypeptide of any one of claims 5 - 15 and a pharmaceutically acceptable carrier.
17. An immunogenic composition comprising the composition claim 16 and an adjuvant. claim 16 and an adjuvant.