Combination of immuno gene therapy and chemotherapy for treatment of cancer and hyperproliferative diseases
Pharmaceutical compositions comprising a nucleic acid, a gene delivery polymer, and at least one adjunctive chemotherapeutic drug for the treatment of mammalian cancer or hyperproliferative disorders and methods of using thereof for the treatment of mammalian cancer or hyperproliferative disorders by intratumoral, intraperitoneal or systemic injection.
1. A method of treating a hyperproliferative disorder in a subject in need thereof comprising administering a pharmaceutical composition comprising: (1) a DNA plasmid that encodes an anti-proliferative molecule selected from the group consisting of interleukin-2, interleukin-4, interleukin-7, IL-15, interferon-α, interferon-β, interferon-γ, colony stimulating factor, granulocyte-macrophage colony stimulating factor, anti-proliferative anti-angiogenic agents, TNF-α, eNOS, iNOS, IP10, p16, thymidine kinase, p53, Fas-ligand, anti-proliferative bacterial antigens, anti-proliferative viral antigens, anti-proliferative tumor antigens and any combination thereof; and (2) a lipopolymer, wherein the lipopolymer comprises a polyethylenimine backbone, wherein cholesterol and polyethylene glycol are each covalently linked to the polyethylenimine backbone; to the subject, wherein the anti-proliferative molecule is expressed in the subject such that a hyperproliferative disorder is treated.
2. The method of claim 1 , further comprising administering at least one chemotherapeutic or anticancer agent to the subject.
3. The method of claim 1 , wherein the hyperproliferative disorder is cancer.
4. The method of claim 1 , wherein the pharmaceutical composition provides a systemic effect in the subject.
5. A method of delivering an anti-proliferative molecule to hyperproliferative cells comprising contacting the hyperproliferative cells with a pharmaceutical composition comprising (1) a DNA plasmid that encodes an anti-proliferative molecule selected from the group consisting of interleukin-2, interleukin-4, interleukin-7, IL-15, interferon-α, interferon-β, interferon-γ, colony stimulating factor, granulocyte-macrophage colony stimulating factor, anti-proliferative anti-angiogenic agents, TNF-α, eNOS, iNOS, IP10, p16, thymidine kinase, p53, Fas-ligand, anti-proliferative bacterial antigens, anti-proliferative viral antigens, anti-proliferative tumor antigens and any combination thereof and (2) a lipopolymer, wherein the lipopolymer comprises a polyethylenimine backbone, wherein cholesterol and polyethylene glycol are each covalently linked to the polyethylenimine backbone, wherein the anti-proliferative molecule is expressed in the hyperproliferative cells resulting in a decrease of proliferation of the hyperproliferative cells.
6. The method of claim 5 , further comprising administering at least one chemotherapeutic or anticancer agent to the hyperproliferative cells.
7. The method of claim 1 , wherein the anti-proliferative molecule is a protein or an shRNA.
8. The method of claim 7 , wherein the anti-proliferative protein is a cytokine.
9. The method of claim 1 , wherein the polyethylenimine backbone has a linear or branch configuration with a molecular weight of 100-500,000 Daltons.
10. The method of claim 1 , wherein the cholesterol is attached to the polyethylenimine backbone through a polyethylene glycol spacer.
11. The method of claim 1 , wherein the polyethylene glycol has molecular weight of between 50 to 20,000 Daltons.
12. The method of claim 1 , wherein the molar ratio of polyethylene glycol to polyethylenimine is within a range of 0.1:1 to 500:1.
13. The method of claim 1 , wherein molar ratio of the cholesterol to polyethylenimine is within a range of 0.1:1 to 500:1.
14. The method of claim 1 , wherein the polyethylenimine backbone further comprises a targeting moiety, wherein the targeting moiety is directly attached to the polyethylenimine backbone or is attached to the polyethylenimine backbone through a polyethylene glycol linker.
15. The method of claim 14 , wherein the molar ratio of the lipopolymer to targeting moiety is within a range of 1:0.1 to 1:100.
16. The method of claim 1 , wherein the DNA plasmid is complexed with the lipopolymer at nitrogen moles in the lipopolymer to phosphate moles in the plasmid DNA at a molar ratio of 0.1:100 to 100:1.
17. The method of claim 5 , wherein the anti-proliferative molecule is a protein or an shRNA.
18. The method of claim 5 , wherein the polyethylenimine backbone has a linear or branch configuration with a molecular weight of 100-500,000 Daltons.
19. The method of claim 5 , wherein the cholesterol is attached to the polyethylenimine backbone through a polyethylene glycol spacer.
20. The method of claim 5 , wherein the polyethylenimine backbone further comprises a targeting moiety, wherein the targeting moiety is directly attached to the polyethylenimine backbone or is attached to the polyethylenimine backbone through a polyethylene glycol linker.