Mutant of the proB gene from coryneform bacteria
View Patent ↗The invention relates to processes for fermentative production of L-proline using bacteria which contain mutated variants of the proB gene.
1. A process for the preparation of L-proline comprising
a) fermenting a host microorganism comprising a polynucleotide encoding a polypeptide comprising an amino acid sequence which is at least 95% identical to the protein of SEQ ID NO: 2 wherein the amino acid sequence does not have glycine at position 149 and has γ-glutamyl kinase activity,
b) enrichment of said L-proline in the medium or in the cells of the microorganism and
c) isolating or collecting said L-proline.
2. The process according to claim 1 , wherein the host microorganism comprises a polynucleotide encoding a polypeptide according to SEQ ID NO: 2 which does not has glycine at position 149 and has γ-glutamyl kinase activity.
3. The process according to claim 2 , wherein position 149 is L-aspartic acid.
4. The process according to claim 1 , wherein the polynucleotide comprises SEQ ID NO: 3 or encodes SEQ ID NO: 4.
5. The process according to claim 2 , wherein the polynucleotide comprises SEQ ID NO: 3 or encodes SEQ ID NO: 4.
6. The process according to claim 3 , wherein the polynucleotide comprises SEQ ID NO: 3 or encodes SEQ ID NO: 4.
7. The process according to claim 1 , wherein the polynucleotide is overexpressed by increasing the copy number of said polynucleotide or by using a strong promoter.
8. The process according to claim 2 , wherein the polynucleotide is overexpressed by increasing the copy number of said polynucleotide or by using a strong promoter.
9. The process according to claim 3 , wherein the polynucleotide is overexpressed by increasing the copy number of said polynucleotide or by using a strong promoter.
10. The process according to claim 4 , wherein the polynucleotide is overexpressed by increasing the copy number of said polynucleotide or by using a strong promoter.
11. The process according to claim 5 , wherein the polynucleotide is overexpressed by increasing the copy number of said polynucleotide or by using a strong promoter.
12. The process according to claim 6 , wherein the polynucleotide is overexpressed by increasing the copy number of said polynucleotide or by using a strong promoter.
13. The process according to claim 1 , wherein the host organism is a Corynebacterium.
14. The process according to claim 2 , wherein the host organism is a Corynebacterium.
15. The process according to claim 3 , wherein the host organism is a Corynebacterium.
16. The process according to claim 4 , wherein the host organism is a Corynebacterium.
17. The process according to claim 7 , wherein the host organism is a Corynebacterium.