Polymer conjugates of nesiritide peptides
View Patent ↗The invention provides peptides that are chemically modified by covalent attachment of a water-soluble oligomer. A conjugate of the invention, when administered by any of a number of administration routes, exhibits characteristics that are different from the characteristics of peptide not attached to the water-soluble oligomer.
1. A conjugate comprising a residue of a nesiritide moiety wherein the conjugate has the structure:
wherein each n is an integer from 10 to 1800.
2. The conjugate of claim 1 , wherein each CH 3 O—(CH 2 CH 2 O) n CH 2 CH 2 — has a weight-average molecular weight in a range of from about 2000 Daltons to about 50,000 Daltons.
3. The conjugate of claim 2 , wherein each CH 3 O—(CH 2 CH 2 O) n CH 2 CH 2 — has a weight-average molecular weight in a range of from about 5000 Daltons to about 40,000 Daltons.
4. The conjugate of claim 1 , wherein the nesiritide moiety is attached at an amino-terminal amino acid of the nesiritide moiety.
5. The conjugate of claim 1 , wherein the nesiritide moiety is attached at an epsilon amino group of an internal lysine amino acid of the nesiritide moiety.
6. A pharmaceutical composition comprising a conjugate of claim 1 and a pharmaceutically acceptable excipient.
7. A method for making a conjugate of claim 1 comprising contacting, under conjugation conditions, a nesiritide moiety with a polymeric reagent bearing a functional group.
8. The conjugate of claim 1 , wherein the nesiritide moiety has the amino acid sequence of SEQ ID NO: 1.
9. The conjugate of claim 2 , wherein each CH 3 O—(CH 2 CH 2 O) n CH 2 CH 2 — has a weight-average molecular weight of about 20,000 Daltons.