Micro titre plate
View Patent ↗A micro titer plate comprising a number of wells filled with separation matrix. According to the invention the volume of the separation matrix is varied between at least some of the wells.
1. A micro titre plate comprising a number of wells disposed in a plurality of columns and rows, the wells being filled with the same type of separation matrix having a series of volumes V 1 , V 2 , . . . V n distributed according to a specific pattern of FIG. 1, 2 , or 3 and FIG. 4 .
2. The micro titre plate of claim 1 wherein the series of volumes V 1 , V 2 , . . . V n is distributed according to a specific pattern for the calculation of an adsorption isotherm of the separation matrix such that the series of volumes results in a series of corresponding isotherm data points in the calculation of the adsorption isotherm.
3. The micro titre plate of claim 1 , wherein each well in the plate has a permeable filter in the bottom.
4. A method for determining an adsorption isotherm comprising the steps of:
using the micro titre plate of claim 1 ;
adding the same volume and concentration of a liquid sample to the number of wells needed for the analysis;
waiting for a predefined period of time until at least some of the sample is adsorbed onto the separation matrix; and
measuring concentration of the sample remaining in the liquid phase and/or solid phase in at least two wells with different volumes of separation matrix.
5. The method of claim 4 , wherein the predefined waiting time is until an equilibrium between the liquid and solid phases has been reached.
6. A method for determining an adsorption isotherm comprising the steps of:
using a micro titre plate that comprises a number of wells disposed in a plurality of columns and rows, the wells being filled with the same type of separation matrix having a series of volumes V 1 , V 2 , . . . V n distributed according to a specific pattern of FIG. 1, 2 , or 3 and FIG. 4 ;
adding the same volume and concentration of a liquid sample to the number of wells needed for the analysis;
waiting for a predefined period of time until at least some of the sample is adsorbed onto the separation matrix; and
measuring concentration of the sample remaining in the liquid phase and/or solid phase in at least two wells with different volumes of separation matrix.