Modified amyloid β peptide
View Patent ↗An object of the invention is to provide a peptide based on a sequence of an amyloid β peptide that may allow for induction of enhanced immune response and is safe and efficacious for prophylaxis and treatment of Alzheimer disease. An amyloid β peptide or a portion thereof with addition or insertion of cysteine or a cysteine analogue, and a method for enhancing immune response to amyloid β using said peptide, a medicament for prophylaxis and treatment of Alzheimer disease using said amyloid β peptide that induces an enhanced immune response, and a DNA vaccine comprising a gene coding for an amyloid β peptide or a sequence derived from an amyloid β peptide with addition or insertion of cysteine or a cysteine analogue, as expected to be similarly efficacious.
1. A peptide consisting of peptide (a) and peptide (b), wherein:
peptide (a) is selected from the group consisting of a peptide of the amino acid residues No. 1 to No. 10 from the N-terminal of SEQ ID NO:1, a peptide of the amino acid residues No. 2 to No. 10 from the N-terminal of SEQ ID NO:1, a peptide of the amino acid residues No. 3 to No. 10 from the N-terminal of SEQ ID NO:1, a peptide of the amino acid residues No. 1 to No. 9 from the N-terminal of SEQ ID NO:1, a peptide of the amino acid residues No. 2 to No. 9 from the N-terminal of SEQ ID NO:1, and a peptide of the amino acid residues No. 1 to No. 18 from the N-terminal of SEQ ID NO:1, and
peptide (b) is selected from the group consisting of a peptide of the amino acid residues No. 28 to No. 36 from the N-terminal of SEQ ID NO:1, a peptide of the amino acid residues No. 28 to No. 37 from the N-terminal of SEQ ID NO:1, a peptide of the amino acid residues No. 28 to No. 38 from the N-terminal of SEQ ID NO:1, a peptide of the amino acid residues No. 28 to No. 39 from the N-terminal of SEQ ID NO:1, a peptide of the amino acid residues No. 28 to No. 40 from the N-terminal of SEQ ID NO:1, a peptide of the amino acid residues No. 28 to No. 41 from the N-terminal of SEQ ID NO:1, and a peptide of the amino acid residues No. 28 to No. 42 from the N-terminal of SEQ ID NO:1,
wherein the C-terminal of peptide (a) is bound to the N-terminal of peptide (b), and wherein cysteine or a cysteine analog is bound to the C-terminal of a resulting combination of peptide (a) and peptide (b).
2. The peptide of claim 1 , wherein a cysteine analog is bound to the C-terminal of the resulting combination of peptide (a) and peptide (b), and the cysteine analog is homocysteine.
3. The peptide of claim 1 , wherein the peptide
has a sequence of any one of SEQ ID NO:2 to SEQ ID NO:13.
4. The peptide of claim 1 , wherein the peptide has a sequence of SEQ ID NO:2.
5. The peptide of claim 1 , wherein the peptide has a sequence of SEQ ID NO:3.
6. The peptide of claim 1 , wherein the peptide has a sequence of SEQ ID NO:4.
7. The peptide of claim 1 , wherein the peptide has a sequence of SEQ ID NO:5.
8. The peptide of claim 1 , wherein the peptide has a sequence of SEQ ID NO:6.
9. The peptide of claim 1 , wherein the peptide has a sequence of SEQ ID NO:7.
10. The peptide of claim 1 , wherein the peptide has a sequence of SEQ ID NO:8.
11. The peptide of claim 1 , wherein the peptide has a sequence of SEQ ID NO:9.
12. The peptide of claim 1 , wherein the peptide has a sequence of SEQ ID NO:10.
13. The peptide of claim 1 , wherein the peptide has a sequence of SEQ ID NO:11.
14. The peptide of claim 1 , wherein the peptide has a sequence of SEQ ID NO:12.
15. The peptide of claim 1 , wherein the peptide has a sequence of SEQ ID NO:13.
16. A medicament, comprising, as an active ingredient, the peptide of claim 1 ,
wherein the medicament is suitable for treatment of Alzheimer disease.
17. A method for inducing an immune response to amyloidβ, comprising administering to a subject in need thereof, an effective amount of the peptide of claim 1 .
18. A method for making a medicine, comprising combining the peptide of claim 1 with a pharmaceutically acceptable carrier.