RAPID AND SAFE TECHNIQUE FOR PERFORMING PCR AMPLIFICATIONS
This invention relates to methods for quick and safe identification of pathogens from biological samples. Iodinated resins may be employed to destroy a pathogen while leaving the pathogen's DNA in a state that can be analyzed. The DNA can then serve as a substrate for PCR analysis. The use of these iodinated resins work in a significantly quicker manner than prior art methods and allows scientists to spend a minimal time under Biosafety Level Three (BSL-3) conditions.
1 . A method of analyzing a biological sample comprising the steps of:
a) providing a liquid sample containing a pathogen;
b) contacting the liquid sample with an iodinated resin;
c) separating the iodinated resin from the liquid; and
d) determining the identity of the pathogen through analyzation techniques.
2 . The method of claim 1 in which the iodinated resin is a bead or fragment.
3 . The method of claim 1 in which the contacting of the sample is enhanced with a Vortex apparatus.
4 . The method of claim 3 , wherein the separation of the liquid portion is performed using a centrifuge.
5 . The method of claim 1 , wherein the analyzation technique is PCR.
6 . A method of analyzing a biological sample comprising the steps of:
a) providing a liquid sample containing a pathogen;
b) contacting the liquid sample with an iodinated resin;
c) centrifuging the liquid sample containing the iodinated resin;
d) removing a portion of the liquid sample;
e) adding the portion of the liquid sample to a PCR solution specific for the pathogen; and
f) quantifying the results of the PCR process.
7 . The method of claim 6 , wherein the pathogen is a bacteria or a virus.
8 . The method of claim 6 , wherein the the contacting of the sample is initiated with a Vortex apparatus.
9 . A test kit for carrying out the process according to claim 1 comprising components to amplify nucleic acid.
10 . A kit for analyzing a biological sample for a pathogen comprising a iodinated resin in a sample holder which contains at least enough space to add said biological sample.
11 . The kit of claim 10 , further comprising components to amplify nucleic acid.
12 . A Pre-PCR diagnostic kit comprising an iodinated resin, said kit allowing the elimination of thermal and/or cooling and/or sonification steps and equipment.
13 . A biomolecular DNA/RNA identification kit comprising iodinated resin allowing a PCR equipment to function without thermal and/or cooling and/or sonification equipment.