Microbial enzymes as detergent additives
Provided in part herein are compositions that include isolated microbial enzymes, such as amylases, lipases, and proteases that are useful as detergent additives. Also provided are methods of isolating amylases, lipases, and proteases from microbes, as well as methods of using these enzymes as detergent additives, and for stain removal.
1. A composition comprising a Bacillus strain SV-1 amylase, a Microbacterium strain SRC-010 protease, and a detergent, wherein
the Bacillus strain SV-1 comprises a 16S rDNA sequence comprising SEQ ID NO: 3; and
wherein the Bacillus strain SV-1 has been deposited with the Microbial Type Culture Collection and designated by deposit accession number MTCC5567; and
the Microbacterium strain SRC-010 comprises a 16S rDNA sequence comprising SEQ ID NO: 1; and wherein the Microbacterium strain SRC-010 has been deposited with the Microbial Type Culture Collection and designated by deposit accession number MTCC5565.
2. The composition of claim 1 , further comprising an enzyme stabilizer.
3. The composition of claim 1 , further comprising a surfactant.
4. The composition of claim 1 , further comprising a lipase.
5. The composition of claim 4 , wherein
the lipase is an Acinetobacter strain BS lipase; and
the Acinetobacter strain BS comprises a 16S rDNA sequence comprising SEQ ID NO: 2; or
the Acinetobacter strain BS has been deposited with the Microbial Type Culture Collection and designated by deposit accession number MTCC5563.
6. A composition comprising:
a Bacillus strain SV-1 amylase, wherein the Bacillus strain SV-1 comprises a 16S rDNA sequence comprising SEQ ID NO: 3 and the Bacillus strain SV-1 is deposited with the Microbial Type Culture Collection and designated by deposit accession number MTCC5567;
an Acinetobacter strain BS lipase, wherein the Acinetobacter strain BS comprises a 16S rDNA sequence comprising SEQ ID NO: 2 and the Acinetobacter strain BS is deposited with the Microbial Type Culture Collection and designated by deposit accession number MTCC5563;
a Microbacterium strain SRC-010 protease, wherein the Microbacterium strain SRC-010 comprises a 16S rDNA sequence comprising SEQ ID NO: 1 and the Microbacterium strain SRC-010 is deposited with the Microbial Type Culture Collection and designated by deposit accession number MTCC5565; and
a detergent.
7. The composition of claim 6 , further comprising a surfactant.
8. The composition of claim 6 , further comprising an enzyme stabilizer.