Lipochito-oligosaccharides stimulating arbuscular mycorrhizal symbiosis
View Patent ↗The invention relates to lipochitooligosaccharides obtainable from arbuscular mycorrhizal fungi, and which are useful for stimulating arbuscular mycorrhizal symbiosis, and lateral root formation.
1. A method for stimulating mycorrhization of a plant which comprises the steps of:
contacting a plant, plant part, seedling or seed thereof with one or more lipochitooligosaccharide defined by formula (I):
wherein n is 2 or 3, R 1 represents a saturated or monounsaturated fatty acid chain containing 16 or 18 carbon atoms, and R 2 represents H or SO 3 H; and contacting said plant, plant part, seedling or seed thereof with an arbuscular mycorrhizal fungus.
2. The method of claim 1 , wherein the one or more lipochitooligosaccharide is selected from
a lipochitooligosaccharide of formula (I) wherein R 1 represents a saturated or monounsaturated fatty acid chain containing 18 carbon atoms, and R 2 represents H or SO 3 H.
3. The method of claim 2 , wherein R 1 is a monounsaturated fatty acid chain containing 18 carbon atoms.
4. The method of claim 1 wherein R 1 represents a saturated or monounsaturated fatty acid chain containing 18 carbon atoms, and R 2 represents H.
5. The method of claim 1 wherein R 1 represents a monounsaturated fatty acid chain containing 18 carbon atoms and R 2 represents H.
6. The method of claim 1 , wherein the one or more lipochitooligosaccharide of formula (I) is used at a concentration of 10 −5 to 10 −12 M.
7. The method of claim 1 , wherein the one or more lipochitooligosaccharide of formula (I) is used at a concentration of 10 −7 to 10 −1 ° M.
8. The method of claim 1 , wherein the plant is a legume.
9. The method of claim 1 , wherein the plant is a non-legume.
10. The method of claim 1 , wherein root system development of the plant is stimulated after said steps.
11. The method of claim 1 , wherein mycorrhization of the plant is stimulated after said steps.
12. The method of claim 1 , wherein the one or more lipochitooligosaccharide is an additive for production of an arbuscular mycorrhizal inoculant.
13. A method for stimulating mycorrhization of a plant which comprises the steps of:
contacting a plant, plant part, seedling or seed thereof with mixture of lipochitooligosaccharides defined by formula (I):
wherein n is 2 or 3, R 1 represents a saturated or monounsaturated fatty acid chain containing 16 or 18 carbon atoms, and R 2 represents H or SO 3 H; and
contacting said plant, plant part, seedling or seed thereof with an arbuscular mycorrhizal fungus, wherein the mixture of a lipochitooligosaccharides comprises a lipochitooligosaccharide wherein R 2 represents H, and a lipochitooligosaccharide wherein R 2 represents SO 3 H is uccd.
14. The method of claim 13 , wherein said mixture comprises a lipochitooligosaccharide of formula (I) wherein n=2, R 1 represents a saturated or monounsaturated fatty acid chain containing 18 carbon atoms, and R 2 represents H, and at least one lipochitooligosaccharide selected from:
(a) a lipochitooligosaccharide of formula (I) wherein n=2, R 1 represents a saturated or monounsaturated fatty acid chain containing 18 carbon atoms, and R 2 represents SO 3 H;
(b) a lipochitooligosaccharide of formula (I) wherein n=2, R 1 represents a saturated or monounsaturated fatty acid chain containing 16 carbon atoms, and R 2 represents SO 3 H;
(c) a lipochitooligosaccharide of formula (I) wherein n=3, R 1 represents a saturated or monounsaturated fatty acid chain containing 18 carbon atoms, and R 2 represents SO 3 H; or
(d) a lipochitooligosaccharide of formula (I) wherein n=3, R 1 represents a saturated or monounsaturated fatty acid chain containing 16 carbon atoms, and R 2 represents SO 3 H.
15. The method of claim 14 , wherein said mixture further comprises at least one lipochitooligosaccharide selected from:
(a) a lipochitooligosaccharide of formula (I) wherein n=2, R 1 represents a saturated or monounsaturated fatty acid chain containing 16 carbon atoms, and R 2 represents H;
(b) a lipochitooligosaccharide of formula (I) wherein n=3, R 1 represents a saturated or monounsaturated fatty acid chain containing 16 carbon atoms, and R 2 represents H; or
(c) a lipochitooligosaccharide of formula (I) wherein n=3, R 1 represents a saturated or monounsaturated fatty acid chain containing 18 carbon atoms, and R 2 represents H.
16. A method for stimulating root system development of a plant which comprises contacting a plant, plant part, seedling or seed thereof with a mixture of a lipochitooligosaccharide of formula:
and a lipochitooligosaccharide of formula:
wherein n is 2 or 3 and R 1 represents a saturated or monounsaturated fatty acid chain containing 16 or 18 carbon atoms.
17. The method of claim 16 , wherein the plant is a non-legume plant.