Optical system for multiple reactions
View Patent ↗The invention relates to optical systems and methods for measurement of samples in multiple sample vessels using an array of light sources where the light from the light sources is divided into multiple light beams, and each beam is directed to a sample vessel.
1. An apparatus for measuring the optical properties of a samples in sample vessels, the apparatus comprising:
(a) an array of n light sources;
(b) a holder adapted to mate with a sample plate comprising m sample vessels, wherein the holder adapted to mate with the sample plate comprises a heating element in thermal contact with the sample vessels;
(c) an optical divider comprising a prismatic optical divider and arranged such that light from each light source is divided into x light beams wherein each of the light beams comprise the same wavelength of light; and
(d) a set of source optical components that deliver the light beams from the optical divider to the m sample vessels.
2. The apparatus of claim 1 wherein the light sources comprise light emitting diodes (LEDs).
3. The apparatus of claim 1 wherein m/n=2.
4. The apparatus of claim 1 wherein m/n=4.
5. The apparatus of claim 1 further comprising an optical detector and a set of detector optical components arranged such that light from the m sample vessels is detected by the detector.
6. The apparatus of claim 5 wherein the source optical components comprise an excitation filter and the detector optical components comprise an emission filter.
7. A method for measuring the optical properties of samples, the method comprising:
a) heating and cooling the samples to perform polynucleotide amplification; and
b) measuring the optical properties of the samples during the amplification in order to determine the level of amplification, wherein the measuring of the optical properties of the samples comprises:
i) illuminating samples in an array of sample vessels with an array of n light sources, wherein a holder is adapted to mate with a sample plate which includes the array of sample vessels, and wherein light from the n light sources is divided into x light beams with a prismatic optical divider, such that n times x is m, and m light beams are produced;
ii) directing the m beams to the m sample vessels using optical components; and
iii) measuring light from the m sample vessels at a detector.
8. The method of claim 7 wherein the samples comprise a fluorescent agent, wherein the beams excite the fluorescent agent to produce fluorescent light, and wherein the light measured at the detector comprises said fluorescent light.
9. The method of claim 7 wherein the polynucleotide amplification comprises real time polymerase chain reaction.
10. The method of claim 9 , wherein the samples comprise a fluorescent agent, wherein the beams excite the fluorescent agent to produce fluorescent light, and wherein the light measured at the detector comprises said fluorescent light.
11. An apparatus for measuring the optical properties of samples in sample vessels, the apparatus comprising:
a) an array of n light sources;
b) a holder adapted to mate with a sample plate comprising m sample vessels;
c) an optical divider comprising a prismatic optical divider and arranged such that light from each light source is divided into x light beams wherein each of the light beams comprise the same wavelength of light;
d) a set of source optical components that deliver the light beams from the optical divider to the m sample vessels; and
e) an optical detector and a set of detector optical components arranged such that light from the m sample vessels is detected by the detector; wherein the source optical components comprise an excitation filter and the detector optical components comprise an emission filter.
12. The apparatus of claim 11 , wherein the light sources comprise light emitting diodes (LEDs).
13. The apparatus of claim 11 , wherein m/n=2.
14. The apparatus of claim 11 , wherein m/n=4.
15. The apparatus of claim 1 , wherein the prismatic optical divider comprises one or more prismatic ridges.
16. The apparatus of claim 15 , wherein the one or more prismatic ridges are located on one surface of the prismatic optical divider.
17. The apparatus of claim 15 , wherein the one or more prismatic ridges are located on both an upper surface and a lower surfaces of the prismatic optical divider.
18. The apparatus of claim 11 , wherein the prismatic optical divider comprises one or more prismatic ridges.
19. The apparatus of claim 18 , wherein the one or more prismatic ridges are located on one surface of the prismatic optical divider.
20. The apparatus of claim 18 , wherein the one or more prismatic ridges are located on both an upper surface and a lower surfaces of the prismatic optical divider.
21. The apparatus of claim 11 wherein the holder adapted to mate with the sample plate comprises a heating element in thermal contact with the sample vessels.