Method for producing poly-3-hydroxyalkanoic acid and agglomerates thereof
View Patent ↗When industrially separating and purifying poly-3-hydroxyalkanoic acid produced by a microorganism, to obtain poly-3-hydroxyalkanoic acid agglomerates having an arbitrary volume mean particle diameter with favorable productivity and with decreased amount of an organic solvent used is enabled while decreasing contaminants derived from constitutive components of cellular bodies. According to the present invention, agglomerates of poly-3-hydroxyalkanoic acid are obtained by adjusting the pH of an aqueous poly-3-hydroxyalkanoic acid suspension to fall within an acidic region.
1. A method for producing poly-3-hydroxyalkanoic acid agglomerates, the method comprising
culturing a microorganism having an ability to intracellularly produce poly-3-hydroxyalkanoic acid;
purifying poly-3-hydroxyalkanoic acid in the absence of any organic solvent by degradation and/or removal of impurities to obtain an aqueous poly-3-hydroxyalkanoic acid suspension having an amount of organic nitrogen of not greater than 6,000 ppm per weight of the poly-3-hydroxyalkanoic acid,
allowing poly-3-hydroxyalkanoic acid to be aggregated by adjusting the pH of the aqueous poly-3-hydroxyalkanoic acid suspension to fall within an acidic region after purifying poly-3-hydroxyalkanoic acid by degradation and/or removal of impurities to obtain poly-3-hydroxyalkanoic acid agglomerates.
2. The method for producing poly-3-hydroxyalkanoic acid agglomerates according to claim 1 , wherein the acidic region is a region of the pH being not less than 2.
3. The method for producing poly-3-hydroxyalkanoic acid agglomerates according to claim 2 , wherein the acidic region is a region of the pH being not less than 3.
4. The method for producing poly-3-hydroxyalkanoic acid agglomerates according to claim 1 , wherein the amount of organic nitrogen present in the aqueous poly-3-hydroxyalkanoic acid suspension is not greater than 4,000 ppm per weight of the poly-3-hydroxyalkanoic acid.
5. The method for producing poly-3-hydroxyalkanoic acid agglomerates according to claim 1 , wherein the solvent included in the aqueous poly-3-hydroxyalkanoic acid suspension comprises water, an organic solvent that is miscible with water, or a mixed solvent of water and the organic solvent.
6. The method for producing poly-3-hydroxyalkanoic acid agglomerates according to claim 1 , wherein the poly-3-hydroxyalkanoic acid is a copolymer constituted with two or more types of 3-hydroxyalkanoic acid selected from the group consisting of 3-hydroxypropionate, 3-hydroxybutyrate, 3-hydroxyvalerate, 3-hydroxyhexanoate, 3-hydroxyheptanoate, and 3-hydroxyoctanoate.
7. The method for producing poly-3-hydroxyalkanoic acid agglomerates according to claim 6 , wherein the poly-3-hydroxyalkanoic acid is a binary copolymer of 3-hydroxyhexanoate and 3-hydroxybutyrate, or a ternary copolymer of 3-hydroxyhexanoate, 3-hydroxybutyrate and 3-hydroxyvalerate.
8. The method for producing poly-3-hydroxyalkanoic acid agglomerates according to claim 1 , wherein the microorganism is a microorganism belonging to genus Aeromonas , genus Alcaligenes , genus Ralstonia , or genus Cupriavidus.
9. The method for producing poly-3-hydroxyalkanoic acid agglomerates according to claim 8 , wherein the microorganism is Cupriavidus necator.
10. The method for producing poly-3-hydroxyalkanoic acid agglomerates according to claim 1 , wherein the microorganism is a transformant.
11. The method for producing poly-3-hydroxyalkanoic acid agglomerates according to claim 10 , wherein the microorganism is a transformant into which at least one selected from a poly-3-hydroxyalkanoic acid synthase gene derived from Aeromonas caviae and a variant thereof was introduced.
12. The method for producing poly-3-hydroxyalkanoic acid agglomerates according to claim 2 , wherein the solvent included in the aqueous poly-3-hydroxyalkanoic acid suspension comprises water, an organic solvent that is miscible with water, or a mixed solvent of water and the organic solvent.
13. The method for producing poly-3-hydroxyalkanoic acid agglomerates according to claim 3 , wherein the solvent included in the aqueous poly-3-hydroxyalkanoic acid suspension comprises water, an organic solvent that is miscible with water, or a mixed solvent of water and the organic solvent.
14. The method for producing poly-3-hydroxyalkanoic acid agglomerates according to claim 4 , wherein the solvent included in the aqueous poly-3-hydroxyalkanoic acid suspension comprises water, an organic solvent that is miscible with water, or a mixed solvent of water and the organic solvent.
15. The method for producing poly-3-hydroxyalkanoic acid agglomerates according to claim 1 , wherein the poly-3hydroxyalkanoic acid agglomerates have an amount of organic nitrogen of not greater than 500 ppm.