Treatment for gastrointestinal disorders using a selective, site-activated binding system
The teachings provided herein generally relate to site-activated binding systems that selectively increase the bioactivity of phenolic compounds at target sites. More particularly, the systems taught here include a phenolic compound bound to a reactive oxygen species, wherein the phenolic compound and the reactive oxygen species react at a target area in the presence of an oxidoreductase enzyme.
1 . A method of treating a gastrointestinal condition, the method comprising:
administering an effective amount of a binding system to a damaged tissue of the subject, the binding system comprising
a phenolic compound component comprising a tannin having a molecular weight ranging from about 500 Daltons to about 4000 Daltons; and,
a reactive oxygen species component comprising hydrogen peroxide;
wherein,
the hydrogen peroxide is releasably bound to the tannin at a tannin:peroxide weight ratio that ranges from about 1:1000 to about 10:1;
the binding system is bioactivated at a target site having an oxidoreductase enzyme that is expressed in response to a tissue damage of a subject;
the phenolic compound component binds to the target site selectively, the target site consisting of the damaged tissue; and,
the binding system contains no, or substantially no, unbound hydrogen peroxide prior to the bioactivating at the target site;
wherein the binding system functions as an antitoxin, an anti-inflammatory, or an antimicrobial when bioactivated at the target site of a damaged tissue and assists in the healing of the damaged tissue by inactivating compounds that promote the condition at the target site.
2 . The method of claim 1 , wherein the phenolic compound component comprises a hydrolysable tannin, a condensed tannin, or a combination thereof.
3 . The method of claim 1 , wherein the phenolic compound component comprises a flavanol.
4 . The method of claim 1 , wherein the phenolic compound component comprises a catechin.
5 . The method of claim 1 , wherein the phenolic compound component comprises gallic acid, epigallic acid, or a combination thereof.
6 . The method of claim 1 , wherein the weight ratio of the tannin:peroxide ranges from about 1:1 to about 1:50.
7 . The method of claim 1 , wherein the gastrointestinal disorder is irritable bowel syndrome.
8 . The method of claim 1 , wherein the gastrointestinal disorder is inflammatory bowel disease.
9 . The method of claim 1 , wherein the gastrointestinal disorder is food poisoning.
10 . A method of treating acute diarrhea in a subject, comprising:
orally administering an effective amount of a binding system to a damaged tissue of the subject, the binding system comprising
a phenolic compound component comprising a tannin having a molecular weight ranging from about 500 Daltons to about 4000 Daltons; and,
a reactive oxygen species component comprising hydrogen peroxide;
wherein,
the hydrogen peroxide is releasably bound to the tannin at a tannin:peroxide weight ratio that ranges from about 1:1000 to about 10:1;
the binding system is bioactivated at a target site having an oxidoreductase enzyme that is expressed in response to a tissue damage of a subject;
the phenolic compound component binds to the target site selectively, the target site consisting of the damaged tissue; and,
the binding system contains no, or substantially no, unbound hydrogen peroxide prior to the bioactivating at the target site;
wherein, the binding system prevents, inhibits, or ameliorates a symptom of acute diarrhea in the subject when compared to a second subject in a control group in which the binding system was not administered.
11 . The method of claim 10 , wherein the symptom is selected from the group consisting of a stool score, heartburn, indigestion, urgency of defecation, nausea, vomiting, stomach pain, and bloating.
12 . The method of claim 10 , wherein the phenolic compound component comprises a hydrolysable tannin, a condensed tannin, or a combination thereof.
13 . The method of claim 10 , wherein the phenolic compound component comprises a flavanol.
14 . The method of claim 10 , wherein the phenolic compound component comprises a catechin.
15 . The method of claim 10 , wherein the phenolic compound component comprises gallic acid, epigallic acid, or a combination thereof.
16 . The method of claim 10 , wherein the weight ratio of the tannin:peroxide ranges from about 1:1 to about 1:50.
17 . A method of promoting weight gain in a subject, comprising:
orally administering an effective amount of a binding system to a damaged tissue of the subject, the binding system comprising:
a phenolic compound component comprising a tannin having a molecular weight ranging from about 500 Daltons to about 4000 Daltons; and,
a reactive oxygen species component comprising hydrogen peroxide;
wherein,
the hydrogen peroxide is releasably bound to the tannin at a tannin:peroxide weight ratio that ranges from about 1:1000 to about 10:1;
the binding system is bioactivated at a target site having an oxidoreductase enzyme that is expressed in response to a tissue damage of a subject;
the phenolic compound component binds to the target site selectively, the target site consisting of the damaged tissue; and,
the binding system contains no, or substantially no, unbound hydrogen peroxide prior to the bioactivating at the target site;
wherein, the binding system increases the feed conversion ratio of the subject when compared to a second subject in a control group in which the binding system was not administered.
18 . The method of claim 17 , wherein the symptom is selected from the group consisting of a stool score, heartburn, indigestion, urgency of defecation, nausea, vomiting, stomach pain, and bloating.
19 . The method of claim 17 , wherein the phenolic compound component comprises a hydrolysable tannin, a condensed tannin, or a combination thereof.
20 . The method of claim 17 , wherein the phenolic compound component comprises a flavanol.
21 . The method of claim 17 , wherein the phenolic compound component comprises a catechin.
22 . The method of claim 17 , wherein the phenolic compound component comprises gallic acid, epigallic acid, or a combination thereof.
23 . The method of claim 17 , wherein the weight ratio of the tannin:peroxide ranges from about 1:1 to about 1:50.
24 . A method of improving or maintaining the gastrointestinal health of in a subject, comprising:
orally administering a binding system that selectively increases the bioactivity of phenolic compounds at a target site in the gastrointestinal tract, the system comprising:
a binding molecule component comprising a tannin having a molecular weight ranging from about 500 Daltons to about 4000 Daltons; and,
a reactive oxygen species component comprising hydrogen peroxide;
wherein,
the hydrogen peroxide is releasably bound to the tannin at a tannin:peroxide weight ratio that ranges from about 1:1000 to about 10:1;
the binding system is bioactivated at the target site, the target site having an oxidoreductase enzyme;
the binding molecule binds to the target site; and,
the binding system contains no, or substantially no, unbound hydrogen peroxide prior to the administering.
wherein, the binding system improves the gastrointestinal health in the subject when compared to a second subject in a control group in which the binding system was not administered.
25 . The method of claim 24 , wherein the gastrointestinal health is measured by the amelioration of a symptom selected from the group consisting of a stool score, heartburn, indigestion, urgency of defecation, nausea, vomiting, stomach pain, and bloating.
26 . The method of claim 24 , wherein the gastrointestinal health is measured using a food conversion ratio.