Antimicrobial system for selective, site-activated binding at target sites
The teachings provided herein generally relate to site-activated binding systems that selectively increase the bioactivity of phenolic compounds at target sites. More particularly, the systems taught here include a phenolic compound bound to a reactive oxygen species, wherein the phenolic compound and the reactive oxygen species react at a target area in the presence of an oxidoreductase enzyme.
1 . A method of treating a damaged dermal, mucosal, or gastrointestinal tissue, the method comprising:
administering an effective amount of a binding system to a damaged tissue of the subject, the binding system comprising
a phenolic compound component comprising a tannin having a molecular weight ranging from about 500 Daltons to about 4000 Daltons; and,
a reactive oxygen species component comprising hydrogen peroxide;
wherein,
the hydrogen peroxide is releasably bound to the tannin at a tannin:peroxide weight ratio that ranges from about 1:1000 to about 10:1;
the binding system is bioactivated at a target site having an oxidoreductase enzyme that is expressed in response to a tissue damage of a subject;
the phenolic compound component binds to the target site selectively, the target site consisting of the damaged tissue; and,
the binding system contains no, or substantially no, unbound hydrogen peroxide prior to the bioactivating at the target site;
wherein the binding system functions as an antimicrobial when bioactivated at the target site of the damaged tissue and assists in the healing of the damaged tissue by inactivating toxic compounds at the target site.
2 . The method of claim 1 , wherein the phenolic compound component comprises a hydrolysable tannin, a condensed tannin, or a combination thereof.
3 . The method of claim 1 , wherein the phenolic compound component comprises a flavanol.
4 . The method of claim 1 , wherein the phenolic compound component comprises a catechin.
5 . The method of claim 1 , wherein the phenolic compound component comprises gallic acid, epigallic acid, or a combination thereof.
6 . The method of claim 1 , wherein the weight ratio of the tannin:peroxide ranges from about 1:1 to about 1:50.
7 . The method of claim 1 , wherein the damaged tissue is a dermal tissue.
8 . The method of claim 1 , wherein the damaged tissue is a mucosal tissue.
9 . The method of claim 1 , wherein the damaged tissue is a gastrointestinal tissue.
10 . A method of treating a damaged dermal, mucosal, or gastrointestinal tissue, the method comprising:
administering an effective amount of a binding system to a damaged tissue of the subject, the binding system comprising
a phenolic compound comprising a pomegranate extract and a green tea extract; and, hydrogen peroxide;
wherein, the ratio of phenolic compound to hydrogen peroxide ranges-from about 1:2 to about 1:20 on a molar weight basis
the binding system is bioactivated at a target site having a peroxidase or oxidase that is expressed in response to a tissue damage;
the phenolic compound component binds to the target site selectively, the target site consisting of the damaged tissue; and,
the binding system contains no, or substantially no, unbound hydrogen peroxide prior to the bioactivating at the target site;
wherein the binding system functions as an antimicrobial when bioactivated at the target site of the damaged tissue and assists in the healing of the damaged tissue by inactivating toxic compounds at the target site.
11 . The method of claim 10 , wherein the phenolic compound component comprises a flavanol.
12 . The method of claim 10 , wherein the phenolic compound component comprises a catechin.
13 . The method of claim 10 , wherein the phenolic compound component comprises gallic acid, epigallic acid, or a combination thereof.
14 . The method of claim 10 , wherein the damaged tissue is a dermal tissue.
15 . The method of claim 10 , wherein the damaged tissue is a mucosal tissue.
16 . The method of claim 10 , wherein the damaged tissue is a gastrointestinal tissue.
17 . A method of treating a damaged dermal, mucosal, or gastrointestinal tissue, the method comprising:
administering an effective amount of a binding system to a damaged tissue of the subject, the binding system comprising
a phenolic compound component comprising a tannin having a molecular weight ranging from about 500 Daltons to about 4000 Daltons; and,
a reactive oxygen species component comprising hydrogen peroxide;
wherein,
the hydrogen peroxide is releasably bound to the tannin at a tannin:peroxide weight ratio that ranges from about 1:1 to about 1:50;
the binding system is bioactivated at a target site having an oxidoreductase enzyme that is expressed in response to a tissue damage of a subject, the oxidoreductase enzyme comprising a peroxidase or oxidase;
the phenolic compound component binds to a target site selectively, the target site consisting of the damaged tissue; and,
the binding system contains no, or substantially no, unbound hydrogen peroxide prior to the bioactivating at the target site;
wherein the binding system functions as an antimicrobial when bioactivated at the target site of the damaged tissue and assists in the healing of the damaged tissue by inactivating toxic compounds at the target site.
18 . The method of claim 17 , wherein the phenolic compound component comprises a hydrolysable tannin, a condensed tannin, or a combination thereof.
19 . The method of claim 17 , wherein the phenolic compound component comprises a flavanol.
20 . The method of claim 17 , wherein the phenolic compound component comprises a catechin.
21 . The method of claim 17 , wherein the phenolic compound component comprises gallic acid, epigallic acid, or a combination thereof.
22 . The method of claim 17 , wherein the damaged tissue is a dermal tissue.
23 . The method of claim 17 , wherein the damaged tissue is a mucosal tissue.
24 . The method of claim 17 , wherein the damaged tissue is a gastrointestinal tissue.